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Atpase Alpha 1, Na, K Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609424

Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the… More details

$770 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATPase alpha 1, Na, K, Monoclonal Antibody
Full Product Name
ATPase alpha 1, Na, K
Product Synonym Names
Anti -ATPase alpha 1, Na, K
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3C114
Host
Mouse
Species Reactivity
Canine, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
Form/Format
Supplied as a liquid, 0.1% sodium azide.
Purity/Purification
Ascites
Ascities
Specificity
Recognizes rabbit Na,K-ATPase alpha 1 subunit. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified Na,K-ATPase from rabbit renal outer medulla

Product Categories/Family for anti-ATPase alpha 1, Na, K antibody

Antibodies; Abs to Enzymes; ATPase
Applications Tested/Suitable for anti-ATPase alpha 1, Na, K antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ATPase alpha 1, Na, K antibody
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Dilution: Western Blot: 1:1000-1:10,000 Detects a band at ~112kD. Do not boil the sample prior to loading on the gel (60 degree C is sufficient).
Immunohistochemistry (Paraffin): 1:200
Immunofluorescence: 1:100-1:1000

Western Blot (WB) of anti-ATPase alpha 1, Na, K antibody

Western Blot analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein, using MBS609424
Mouse Anti-Atpase Alpha 1, Na, K Monoclonal Antibody – Cat# MBS609424

Immunohistochemistry (IHC) of anti-ATPase alpha 1, Na, K antibody

Immunohistochemistry: Staining of enodmetrial glands within the uterus using MBS609424 Note the absence of staining in the surrounding myometrial smooth muscle.
Mouse Anti-Atpase Alpha 1, Na, K Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS609424

Immunofluorescence (IF) of anti-ATPase alpha 1, Na, K antibody

mmunocytochemistry/Immunofluorescence of HepG2 cells using MBS609424.|Detection of ATPA1 (Green) Nuclei (Blue) were counterstained using Hoechst
Mouse Anti-Atpase Alpha 1, Na, K Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS609424

Flow Cytometry (FC/FACS) of anti-ATPase alpha 1, Na, K antibody

IFlow Cytometry of fixed A549 cells using MBS609424.
Mouse Anti-Atpase Alpha 1, Na, K Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS609424

Other Notes

Small volumes of anti-ATPase alpha 1, Na, K antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATPase alpha 1, Na, K purchase

MBS609424 is an antibody targeting ATPase alpha 1, Na, K. Reactive in Canine, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus. Host: Mouse.
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