Mouse Anti-SIGIRR Monoclonal Antibody – Cat# MBS609464

Flow Cytometry of human PBMCs, unstimulated (A) or activated with PMA/Ca2+ ionomycin/LPS and recombinant human IL-23 (B), were stained with CD3 epsilon (PE) and MBS609464. Quadrant markers were set based upon isotype control staining.

SIGIRR Antibody – Mouse Monoclonal

Name: SIGIRR Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS609464
Price: 1090 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609464

SIGIRR is a type I transmembrane protein belonging to the IL-1 receptor superfamily. It has a single extracellular Ig-like domain and conserved motifs in its cytoplasmic domain that are characteristic of the IL-1 receptor and Toll-like receptor families. More details

$1090 / 100 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SIGIRR, Monoclonal Antibody

Full Product Name

SIGIRR (Single Ig IL-1-related Receptor, Single Ig IL-1R-related Molecule, Single Immunoglobulin Domain-containing IL1R-related Protein, Toll/Interleukin-1 Receptor 8, TIR8, UNQ301/PRO342) (APC)

Product Gene Name

anti-SIGIRR antibody

Product Synonym Names

Anti -SIGIRR (Single Ig IL-1-related Receptor, Single Ig IL-1R-related Molecule, Single Immunoglobulin Domain-containing IL1R-related Protein, Toll/Interleukin-1 Receptor 8, TIR8, UNQ301/PRO342) (APC)

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

10K61

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human SIGIRR.

Preparation and Storage

May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing Allophycocyanin conjugates will result in a substantial loss of enzymatic activity.

Molecular Weight

45,679 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (405716..417397, complement). Location: 11p15.5

OMIM

605478

3D Structure

ModBase 3D Structure for Q6IA17

Immunogen

Recombinant corresponding to human SIGIRR expressed in NSO cells (Q6IA17).

Product Categories/Family for anti-SIGIRR antibody

Antibodies; Abs to Interleukin

Applications Tested/Suitable for anti-SIGIRR antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-SIGIRR antibody

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using human PBMCs.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of S1013-68G to 1-2.5 x 10e5 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2a antibody.

Other Notes

Small volumes of anti-SIGIRR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SIGIRR purchase

MBS609464 is an antibody targeting SIGIRR. Also known as SIGIRR (Single Ig IL-1-related Receptor, Single Ig IL-1R-related Molecule, Single Immunoglobulin Domain-containing IL1R-related Protein, Toll/Interleukin-1 Receptor 8, TIR8, UNQ301/PRO342) (APC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SIGIRR. It may not necessarily be applicable to this product.

NCBI GI #

205277451

NCBI GeneID

59307

NCBI Accession #

NP_001128526.1

UniProt Primary Accession #

Q6IA17

UniProt Secondary Accession #

Q3KQY2; Q6UXI3; Q9H733

UniProt Related Accession #

Q6IA17

GenBank Nucleotide #

NM_001135054.1

NCBI Official Full Name

single Ig IL-1-related receptor

NCBI Official Synonym Full Names

single immunoglobulin and toll-interleukin 1 receptor (TIR) domain

NCBI Official Symbol

SIGIRR

NCBI Official Synonym Symbols

TIR8; MGC110992

UniProt Protein Name

Single Ig IL-1-related receptor

UniProt Synonym Protein Names

Single Ig IL-1R-related molecule; Single immunoglobulin domain-containing IL1R-related protein; Toll/interleukin-1 receptor 8

Protein Family

Single Ig IL-1-related receptor

UniProt Gene Name

SIGIRR

UniProt Entry Name

SIGIR_HUMAN

NCBI Protein Information

single Ig IL-1-related receptor; OTTHUMP00000229641; OTTHUMP00000229642; OTTHUMP00000229643; OTTHUMP00000229644; OTTHUMP00000229645; toll/interleukin-1 receptor 8; single Ig IL-1R-related molecule; single immunoglobulin domain IL1R1 related; single immunoglobulin domain-containing IL1R-related protein

UniProt Comments for SIGIRR

SIGIRR: Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: membrane; integral to membrane

Molecular Function: protein binding

Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of transcription factor activity; acute-phase response; negative regulation of cytokine and chemokine mediated signaling pathway; signal transduction; negative regulation of chemokine biosynthetic process

Research Articles on SIGIRR

1. SIGIRR can inhibit TLR4, 5, and 9-mediated immune responses in human airway epithelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SIGIRR antibody

Reference Product	PubMed Publications
TLR4 antibody	>19 publications with SIGIRR and TLR4
TLR9 antibody	>3 publications with SIGIRR and TLR9
TRAF6 antibody	>1 publications with SIGIRR and TRAF6
TLR5 antibody	>1 publications with SIGIRR and TLR5
IRAK1 antibody	>1 publications with SIGIRR and IRAK1

Pathways associated with anti-SIGIRR antibody

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immunity Signaling Pathway antibodies	Innate Immunity Signaling Pathway Diagram
MyD88:Mal Cascade Initiated On Plasma Membrane Pathway antibodies	MyD88:Mal Cascade Initiated On Plasma Membrane Pathway Diagram
Toll Like Receptor 2 Cascade Pathway antibodies	Toll Like Receptor 2 Cascade Pathway Diagram
Toll Like Receptor 4 (TLR4) Cascade Pathway antibodies	Toll Like Receptor 4 (TLR4) Cascade Pathway Diagram
Toll Like Receptor TLR1:TLR2 Cascade Pathway antibodies	Toll Like Receptor TLR1:TLR2 Cascade Pathway Diagram
Toll Like Receptor TLR6:TLR2 Cascade Pathway antibodies	Toll Like Receptor TLR6:TLR2 Cascade Pathway Diagram
Toll Receptor Cascades Pathway antibodies	Toll Receptor Cascades Pathway Diagram

Diseases associated with anti-SIGIRR antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>27 publications with SIGIRR and Inflammation
Colitis Antibodies	>7 publications with SIGIRR and Colitis
Kidney Diseases Antibodies	>3 publications with SIGIRR and Kidney Diseases
Psoriasis Antibodies	>1 publications with SIGIRR and Psoriasis
Adenoma Antibodies	>1 publications with SIGIRR and Adenoma
Fibrosis Antibodies	>1 publications with SIGIRR and Fibrosis
Influenza, Human Antibodies	>1 publications with SIGIRR and Influenza, Human
Cell Transformation, Neoplastic Antibodies	>1 publications with SIGIRR and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-SIGIRR antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>17 publications with SIGIRR and Blood
Kidney Antibodies	>8 publications with SIGIRR and Kidney
Intestine Antibodies	>8 publications with SIGIRR and Intestine
Lung Antibodies	>8 publications with SIGIRR and Lung
Brain Antibodies	>5 publications with SIGIRR and Brain
Spleen Antibodies	>2 publications with SIGIRR and Spleen
Liver Antibodies	>2 publications with SIGIRR and Liver
Bone Marrow Antibodies	>1 publications with SIGIRR and Bone Marrow
Thymus Antibodies	>1 publications with SIGIRR and Thymus
Heart Antibodies	>1 publications with SIGIRR and Heart