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PHD2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609533

PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. EGLN family, which is known to be cellular oxygen sensor, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can… More details

$655 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PHD2, Monoclonal Antibody
Full Product Name
PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20)
Product Synonym Names
Anti -PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20)
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
10K404
Host
Mouse
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human PHD2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant human PHD2 (aa271-426) purified from E.coli (Accession #NP_071334).
Hybridoma
Mouse F0 myeloma cells with spleen cells from BALB/c mice.

Product Categories/Family for anti-PHD2 antibody

Applications Tested/Suitable for anti-PHD2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-PHD2 antibody
Suitable for use in Immunofluorescence, ELISA, and Western Blot.
Dilution: Immunofluorescence: 1:500-1:1,000
Western Blot:1:500-1:1,000

Immunofluorescence (IF) of anti-PHD2 antibody

Immunofluorescence analysis: HeLa cells were stained with MBS609533 (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
Mouse Anti-PHD2 Monoclonal Antibody – Cat# MBS609533

Western Blot (WB) of anti-PHD2 antibody

Western blot analysis: Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with MBS609533 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-PHD2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS609533

Other Notes

Small volumes of anti-PHD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHD2 purchase

MBS609533 is an antibody targeting PHD2. Also known as PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20). Reactive in Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PHD2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
PHD2
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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