Mouse Anti-CLEC5A Monoclonal Antibody – Cat# MBS609584

Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) S1013-58B and (B) MBS609584 (filled histogram) or isotype control antibody (open histogram).

CLEC5A Antibody – Mouse Monoclonal

Name: CLEC5A Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS609584
Price: 980 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609584

CLEC5A is a type II membrane protein belonging to the C-type lectin superfamily. CLEC5A is expressed on monocytes/macrophages, dendritic cells, and NK cells and contributes to cellular activation through its association with DAP12. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CLEC5A, Monoclonal Antibody

Full Product Name

CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC)

Product Gene Name

anti-CLEC5A antibody

Product Synonym Names

Anti -CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC)

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

11C8

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC)

Purity/Purification

Purified by Protein A affinity chromatography from hybridoma culture supernatant

Concentration

0.01 mg/ml (lot specific)

Specificity

Recognizes human CLEC5A. Does not recognize recombinant mouse CLEC5A

Preparation and Storage

Store at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap

Chromosome Location

Chromosome: 7; NC_000007.13 (141627157..141646783, complement). Location: 7q33

OMIM

604987

3D Structure

ModBase 3D Structure for Q9NY25

Positive Control

Human peripheral blood granulocytes

Immunogen

Recombinant protein corresponding to Tyr26-Lys188 human CLEC5A expressed in NS0 cells

Product Categories/Family for anti-CLEC5A antibody

Antibodies; Abs to Lectins

Applications Tested/Suitable for anti-CLEC5A antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-CLEC5A antibody

Flow Cytometry: 10 ul labels up to 1x10e6 cells

Flow Cytometry Protocol:
1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of to 1-2.5x10e5 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody.

Optimal dilutions to be determined by the researcher

Other Notes

Small volumes of anti-CLEC5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CLEC5A purchase

MBS609584 is an antibody targeting CLEC5A. Also known as CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CLEC5A. It may not necessarily be applicable to this product.

NCBI GI #

10281669

NCBI GeneID

23601

NCBI Accession #

NP_037384.1

UniProt Primary Accession #

Q9NY25

UniProt Secondary Accession #

Q52M11; Q9UKQ0

UniProt Related Accession #

Q14DL9; Q9NY25

GenBank Nucleotide #

NM_013252.2

NCBI Official Full Name

C-type lectin domain family 5 member A

NCBI Official Synonym Full Names

C-type lectin domain family 5, member A

NCBI Official Symbol

CLEC5A

NCBI Official Synonym Symbols

MDL1; MDL-1; CLECSF5; MGC138304

UniProt Protein Name

C-type lectin domain family 5 member A

UniProt Synonym Protein Names

C-type lectin superfamily member 5; Myeloid DAP12-associating lectin 1

Protein Family

C-type lectin domain family

UniProt Gene Name

CLEC5A

UniProt Synonym Gene Names

CLECSF5; MDL1

UniProt Entry Name

CLC5A_HUMAN

NCBI Protein Information

C-type lectin domain family 5 member A; OTTHUMP00000211626; OTTHUMP00000211628; OTTHUMP00000211630; C-type lectin superfamily member 5; myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 5

NCBI Summary for CLEC5A

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq]

UniProt Comments for CLEC5A

CLEC5A: Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: viral receptor activity; carbohydrate binding

Biological Process: negative regulation of myeloid cell apoptosis; entry of virus into host cell; response to virus; innate immune response; cellular defense response; myeloid cell differentiation; signal transduction; osteoblast development; negative regulation of apoptosis; positive regulation of cytokine secretion

Research Articles on CLEC5A

1. CLEC5A expression in monocyte/macrophage and granulocytes is regulated by PU.1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CLEC5A antibody

Reference Product	PubMed Publications
TYROBP antibody	>7 publications with CLEC5A and TYROBP

Diseases associated with anti-CLEC5A antibody

Disease Name	Pubmed Publications
Dengue Hemorrhagic Fever Antibodies	>2 publications with CLEC5A and Dengue Hemorrhagic Fever

Organs/Tissues associated with anti-CLEC5A antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>8 publications with CLEC5A and Blood
Bone Marrow Antibodies	>3 publications with CLEC5A and Bone Marrow
Connective Tissue Antibodies	>1 publications with CLEC5A and Connective Tissue
Brain Antibodies	>1 publications with CLEC5A and Brain
Lung Antibodies	>1 publications with CLEC5A and Lung