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Heat Shock Protein 27 (HSP27) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609607

MBS609607 is an antibody targeting Hsp27. Also known as Heat Shock Protein 27, phosphorylated (Ser78) (HSP27). Reactive in Human. Host: Mouse; Source: Human. More details

$1090 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Heat Shock Protein 27 (Hsp27), Monoclonal Antibody
Full Product Name
Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)
Product Gene Name
Product Synonym Names
Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)
Clonality
Monoclonal
Isotype
IgG
Clone Number
2T7
Host
Host: Mouse; Source: Human
Species Reactivity
Human
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human phosphorylated HSP27 (Ser78). Does not recognize the non-phosphorylated form of HSP27. An additional band was observed at ~54kD in longer exposures. Species Sequence Homology: canine, 100%; Mouse and rat based on GenPept database sequence where Ser78 is replaced with Asp.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 3L; NT_037436.3 (9377165..9378384). Location: 67B3-67B3
Immunogen
Synthetic peptide corresponding to aa73-83 (YSRAL[pS]RQLSS-C) of human HSP27 with a C-terminus cysteine added for conjugation, conjugated to KLH.
Applications Tested/Suitable for anti-Hsp27 antibody
Western Blot (WB)
Application Notes for anti-Hsp27 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 0.01-0.5ug/ml detects phosphorylated HSP27 in 20ug of a cell lysate prepared from MCF-7 cells that were heat shocked for 30 minutes at 42 degree C, cultured for an additional 16 hours at 37 degree C, and then stimulated with 200mM arsenite for 30 minutes. Untreated and heat shock, and arsenite treated MCF-7 cell lysate were resolved by electrophoresis, transferred to nitrocellulose and probed with 0.05ug/ml of H1830-52. Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-Hsp27 antibody

Western Blot of MCF-7 cell lysate uing MBS609607 (0.05ug/ml):|Lane 1: Untreated |Lane 2: Heat shock and arsenite treated|Arrow indicates phosphorylated HSP27.
Mouse Anti-HSP27 Monoclonal Antibody – Cat# MBS609607

Other Notes

Small volumes of anti-Hsp27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp27. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
heat shock protein 27
NCBI Official Synonym Full Names
Heat shock protein 27
NCBI Official Symbol
Hsp27
NCBI Official Synonym Symbols
27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
Protein Family
NCBI Protein Information
CG4466 gene product from transcript CG4466-RA; heat shock protein 27; CG4466-PA; Hsp27-PA; heat shock protein hsp27; Hsp 27

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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