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Semaphorin 4b (SEMA4B) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609683

Semaphorin 4B (Sema4B; also SemaC) is a 95-100kD, class IV member of the semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature human… More details

$1025 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Semaphorin 4B (SEMA4B), Monoclonal Antibody
Full Product Name
Semaphorin 4B (SEMA4B, Semaphorin-4B, KIAA1745, SEMAC, UNQ749/PRO1480)
Product Gene Name
Product Synonym Names
Anti -Semaphorin 4B (SEMA4B, Semaphorin-4B, KIAA1745, SEMAC, UNQ749/PRO1480)
Clonality
Monoclonal
Isotype
IgG2B
Clone Number
10K60
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Semaphorin 4B.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
92,750 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (90728152..90772892). Location: 15q25
3D Structure
ModBase 3D Structure for Q2NL81
Immunogen
Purified, NS0-derived, recombinant human Semaphorin 4B (aa39-712; Accession #Q9NPR2).
Hybridoma
Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Semaphorin 4B.
Applications Tested/Suitable for anti-SEMA4B antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-SEMA4B antibody
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 0.5-1ug/ml

Testing Data of anti-SEMA4B antibody

THP-1 cells were stained with MBS609683 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.
Mouse Anti-SEMA4B Monoclonal Antibody – Cat# MBS609683

Other Notes

Small volumes of anti-SEMA4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEMA4B purchase

MBS609683 is an antibody targeting SEMA4B. Also known as Semaphorin 4B (SEMA4B, Semaphorin-4B, KIAA1745, SEMAC, UNQ749/PRO1480). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA4B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
semaphorin-4B
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
NCBI Official Symbol
SEMA4B
NCBI Official Synonym Symbols
SemC; SEMAC; KIAA1745; MGC131831
UniProt Protein Name
Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (Semaphorin) 4B
Protein Family
UniProt Gene Name
SEMA4B
UniProt Entry Name
Q2NL81_HUMAN
NCBI Protein Information
semaphorin-4B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B

UniProt Comments for SEMA4B

SEMA4B iso3: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Two alternatively spliced human isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 15q25

Cellular Component: plasma membrane; integral to membrane; synapse

Molecular Function: receptor activity

Biological Process: nervous system development; cell differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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