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Kinase P44/42 (MAP) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS609868

MBS609868 is an antibody targeting MAP Kinase p44/42. Also known as MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase… More details

$1045 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAP Kinase p44/42, Monoclonal Antibody
Full Product Name
MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas
Product Synonym Names
Anti -MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
0.T.87
Host
Mouse
Species Reactivity
Chicken, Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, 0.1% sodium azide, 30% glycerol.
Purity/Purification
Highly Purified
Purified by thiophilic absorption followed by size exclusion chromatography.
Specificity
Specific for phosphorylated Erk1 and Erk2; recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2. Does not crossreact with activated SAP Kinase 1 or 2.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2.
Applications Tested/Suitable for anti-MAP Kinase p44/42 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MAP Kinase p44/42 antibody
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Immunoblot Analysis: 0.1-1ug/ml detects pMAPK in RIPA lysates from EGF-stimulated human A431 carcinoma cells. Non-stimulated A431 and EGF-stimulated A431 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-pMAPK (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: Use 1-10ug per immunoprecipitation.

Western Blot (WB) of anti-MAP Kinase p44/42 antibody

Western Blot: A431+EGF cell lysate (lane 2) and unstimulated (lane 1) was probed with MBS609868 at 0.5ug/ml.
Mouse Anti-MAP Monoclonal Antibody – Cat# MBS609868

Other Notes

Small volumes of anti-MAP Kinase p44/42 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP Kinase p44/42. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
MAP kinase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP Kinase p44/42 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with MAP Kinase p44/42 and FLNA
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