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Deoxyribonuclease II (DNASE2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610101

Deoxyribonuclease II (Dnase II, EC.3.1.22.1) is one of the two Dnase present in mammalian tissues and biological fluids. Dnase II hydrolyzes DNA to 3'-phosphoryl oligonucleotides under acidic conditions. Dnase II is also known as "acid Dnase". It has… More details

$965 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Deoxyribonuclease II (DNASE2), Polyclonal Antibody
Full Product Name
Deoxyribonuclease II (DNase II)
Product Gene Name
Product Synonym Names
Anti -Deoxyribonuclease II (DNase II)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography
Specificity
Recognizes mouse Deoxyribonuclease II (Dnase II). The peptide sequence is not well conserved in human Dnase II. No significant sequence homology with other lysosomal enzymes.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
39,581 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (12986025..12992335, complement). Location: 19p13.2
OMIM
126350
3D Structure
ModBase 3D Structure for O00115
Immunogen
A synthetic peptide corresponding to 17aa near the C-terminus of mouse Dnase II, conjugated to KLH
Applications Tested/Suitable for anti-DNASE2 antibody
ELISA (EL/EIA)
Application Notes for anti-DNASE2 antibody
Suitable for use in ELISA.
Dilution: ELISA: 1:10,000-50,000 using 50-100ng control peptide/well.

Other Notes

Small volumes of anti-DNASE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNASE2 purchase

MBS610101 is an antibody targeting DNASE2. Also known as Deoxyribonuclease II (DNase II). Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DNASE2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
deoxyribonuclease II
NCBI Official Synonym Full Names
deoxyribonuclease II, lysosomal
NCBI Official Symbol
DNASE2
NCBI Official Synonym Symbols
DNL; DNL2; DNASE2A
UniProt Protein Name
Deoxyribonuclease-2-alpha
UniProt Synonym Protein Names
Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II; R31240_2
Protein Family
UniProt Gene Name
DNASE2
UniProt Synonym Gene Names
DNASE2A; DNL2
UniProt Entry Name
DNS2A_HUMAN
NCBI Protein Information
deoxyribonuclease-2-alpha; R31240_2; acid DNase; DNase II alpha; lysosomal DNase II; DNase II, lysosomal; deoxyribonuclease II alpha

NCBI Summary for DNASE2

This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq]
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UniProt Comments for DNASE2

DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.

Protein type: EC 3.1.22.1; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; intracellular

Molecular Function: deoxyribonuclease II activity; DNA binding

Biological Process: erythrocyte differentiation; DNA fragmentation during apoptosis; DNA metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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