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CREB, Ser133 (CREB1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610102

The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an… More details

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Product Overview

Online Datasheet in PDF
Product Name
CREB, Ser133 (CREB1), Polyclonal Antibody
Full Product Name
CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)
Product Gene Name
anti-CREB1 antibody
Product Synonym Names
Anti -CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes phosphorylated human CREB at pS133. Species Crossreactivity: mouse and rat. Minimal reactivity with non-phosphorylated CREB by Western Blot and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide. Assayed against a variety of tisues including fibroblasts and B-cell lysates.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
34,877 Da
Chromosome Location
Chromosome: 2; NC_007300.4 (100928794..100961320)
3D Structure
ModBase 3D Structure for P27925
Immunogen
Synthetic peptide corresponding to aa122-147, QKRREILSRRPpSYRKILNDLSSDAP, from human CREB Phosphorylated at pS133 (KLH).

Product Categories/Family for anti-CREB1 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-CREB1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CREB1 antibody
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: Western Blot: 1:500-1:2000. Detects bands of 46 and 43kD corresponding to phosphorylated CREB.
Immunohistochemistry (FFPE): 20ug/ml
ELISA: 1:1000-1:6000

Testing Data of anti-CREB1 antibody

MBS610102 was used to detect phosphorylated CREB by immunoblot. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Immunoblot of indicated amounts of control (-) and in-vitro phosphorylated CREB (P) were loaded to show that the antibody reacts specifically with the phosphorylated form. Blots were blocked in 5% milk in TBS+0.1% Tween-20 (TBST-M) overnight at 4°C. Detection occurs using a 1:500 dilution of antibody diluted in TBST-M and incubated at room temperature with rocking for 1 hour. Blots were rinsed 6X with TBST and incubated with goat anti-rabbit-HRP at 1:5000 in TBST-M at RT for 45 min. Blots were again rinsed 6X with TBST and then processed using ECL reagent (Amersham).
Rabbit Anti-CREB1 Polyclonal Antibody – Cat# MBS610102

Immunohistochemistry (IHC) of anti-CREB1 antibody

Immunohistochemistry. MBS610102 was used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong nuclear staining of tonsillar lymphocytes. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain
Rabbit Anti-CREB1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS610102

Immunoblot (IB) of anti-CREB1 antibody

MBS610102 was used to detect phosphorylated CREB by immunoblot. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Immunoblot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Pan reactive CREB reacts equally with both non-phosphorylated and phosphorylated CREB (not shown). Detection occurs using a 1:500 dilution of antibody followed by 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure was approximately 1’. Other detection systems will yield similar results. Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.
Rabbit Anti-CREB1 Polyclonal Antibody, Immunoblot (IB) – Cat# MBS610102

Other Notes

Small volumes of anti-CREB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CREB1 purchase

MBS610102 is an antibody targeting CREB1. Also known as CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CREB1. It may not necessarily be applicable to this product.

NCBI GI #
27805857
NCBI GeneID
281713
NCBI Accession #
NP_776710.1
UniProt Primary Accession #
P27925
UniProt Secondary Accession #
O18957; A5PK02
UniProt Related Accession #
P27925; Q0V7N6
GenBank Nucleotide #
NM_174285.1
NCBI Official Full Name
cyclic AMP-responsive element-binding protein 1
NCBI Official Symbol
CREB1
NCBI Official Synonym Symbols
CREB
UniProt Protein Name
Cyclic AMP-responsive element-binding protein 1
UniProt Synonym Protein Names
Cyclic AMP-responsive DNA-binding protein
Protein Family
Cyclic AMP-responsive element-binding protein
UniProt Gene Name
CREB1
UniProt Synonym Gene Names
CREB; CREB2
UniProt Entry Name
CREB1_BOVIN
NCBI Protein Information
cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; cyclic AMP-responsive DNA-binding protein

UniProt Comments for CREB1

Function: This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Implicated in synchronization of circadian rhythmicity

By similarity. Ref.4 Ref.5 Ref.6

Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-117, binds CREBBP. Interacts (phosporylated form) with TOX3

Subcellular location: Nucleus.

Post-translational modification: Sumoylated by SUMO1. Sumoylation on Lys-288, but not on Lys-269, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization

By similarity.Stimulated by phosphorylation. Phosphorylation of both Ser-117 and Ser-126 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-117 allows CREBBP binding. Phosphorylated upon DNA damage, probably by ATM or ATR

By similarity. Ref.7

Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP domain.Contains 1 KID (kinase-inducible) domain.
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Research Articles on CREB1

1. dsRNA as well as other agonists of endothelial cells elicit signaling mechanisms that include in part CREB phosphorylation mediated by calcium-independent phospholipase A2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CREB1 antibody

 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 CREB Phosphorylation Pathway antibodies  CREB Phosphorylation Pathway Diagram
 Ca-dependent Events Pathway antibodies  Ca-dependent Events Pathway Diagram
 CaM Pathway antibodies  CaM Pathway Diagram
 CaMK IV-mediated Phosphorylation Of CREB Pathway antibodies  CaMK IV-mediated Phosphorylation Of CREB Pathway Diagram

Diseases associated with anti-CREB1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >109 publications with CREB1 and Neoplasms
 Mental Disorders Antibodies  >54 publications with CREB1 and Mental Disorders
 Skin Diseases Antibodies  >31 publications with CREB1 and Skin Diseases
 Inflammation Antibodies  >31 publications with CREB1 and Inflammation
 Leukemia Antibodies  >30 publications with CREB1 and Leukemia
 Depressive Disorder Antibodies  >26 publications with CREB1 and Depressive Disorder
 Necrosis Antibodies  >25 publications with CREB1 and Necrosis
 Carcinoma Antibodies  >22 publications with CREB1 and Carcinoma
 Breast Neoplasms Antibodies  >19 publications with CREB1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >18 publications with CREB1 and Cardiovascular Diseases

Organs/Tissues associated with anti-CREB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >8 publications with CREB1 and Pancreas
 Ovary Antibodies  >7 publications with CREB1 and Ovary
 Intestine Antibodies  >6 publications with CREB1 and Intestine
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