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Hormone Sensitive Lipase (LIPE) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610150

HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA… More details

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Product Overview

Online Datasheet in PDF
Product Name
Hormone Sensitive Lipase (LIPE), Polyclonal Antibody
Full Product Name
Hormone Sensitive Lipase (HSL)
Product Gene Name
anti-LIPE antibody
Product Synonym Names
Anti -Hormone Sensitive Lipase (HSL)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Specificity
Recognizes endogenous levels of total HSL protein. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
116,598 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (42905666..42931578, complement). Location: 19q13.2
OMIM
151750
3D Structure
ModBase 3D Structure for Q05469
Immunogen
Synthetic peptide derived from the sequence of human HSL (KLH).

Product Categories/Family for anti-LIPE antibody

Antibodies; Abs to Enzymes; Lipase
Applications Tested/Suitable for anti-LIPE antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes for anti-LIPE antibody
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (IF-IC): 1:50

Western Blot (WB) of anti-LIPE antibody

Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells using MBS610150 HSL Antibody.
Rabbit Anti-LIPE Polyclonal Antibody – Cat# MBS610150

Immunofluorescence (IF) of anti-LIPE antibody

Confocal immunofluorescent analysis of 3T3-L1 adipocytes using MBS610150 HSL Antibody (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Rabbit Anti-LIPE Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS610150

Other Notes

Small volumes of anti-LIPE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIPE purchase

MBS610150 is an antibody targeting LIPE. Also known as Hormone Sensitive Lipase (HSL). Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LIPE. It may not necessarily be applicable to this product.

NCBI GI #
21328446
NCBI GeneID
3991
NCBI Accession #
NP_005348.2
UniProt Primary Accession #
Q05469
UniProt Secondary Accession #
Q3LRT2; Q6NSL7
UniProt Related Accession #
Q05469
GenBank Nucleotide #
NM_005357.2
NCBI Official Full Name
hormone-sensitive lipase
NCBI Official Synonym Full Names
lipase, hormone-sensitive
NCBI Official Symbol
LIPE
NCBI Official Synonym Symbols
HSL; LHS
UniProt Protein Name
Hormone-sensitive lipase
Protein Family
Hormone-sensitive lipase
UniProt Gene Name
LIPE
UniProt Entry Name
LIPS_HUMAN
NCBI Protein Information
hormone-sensitive lipase; hormone-sensitive lipase testicular isoform

NCBI Summary for LIPE

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq]
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UniProt Comments for LIPE

HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: Lipase; EC 3.1.1.79

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: lipid particle; caveola; cytosol

Molecular Function: triacylglycerol lipase activity; hormone-sensitive lipase activity; protein binding; protein kinase binding

Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process

Disease: Abdominal Obesity-metabolic Syndrome 4
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Research Articles on LIPE

1. total lipase, ATGL and HSL activities were higher in visceral white adipose tissue of cancer patients compared with individuals without cancer and higher in cancer patients with cachexia compared with cancer patients without cachexia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LIPE antibody

 Reference Product  PubMed Publications
 FABP4 antibody  >2 publications with LIPE and FABP4

Pathways associated with anti-LIPE antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway antibodies  Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Triacylglyceride Synthesis Pathway antibodies  Triacylglyceride Synthesis Pathway Diagram

Diseases associated with anti-LIPE antibody

 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >38 publications with LIPE and Neurodegenerative Diseases
 Obesity Antibodies  >14 publications with LIPE and Obesity
 Congenital Abnormalities Antibodies  >13 publications with LIPE and Congenital Abnormalities
 Neurologic Manifestations Antibodies  >13 publications with LIPE and Neurologic Manifestations
 Nervous System Malformations Antibodies  >11 publications with LIPE and Nervous System Malformations
 Insulin Resistance Antibodies  >7 publications with LIPE and Insulin Resistance
 Drug Toxicity Antibodies  >7 publications with LIPE and Drug Toxicity
 Muscular Diseases Antibodies  >6 publications with LIPE and Muscular Diseases
 Neoplasms Antibodies  >5 publications with LIPE and Neoplasms
 Alzheimer Disease Antibodies  >5 publications with LIPE and Alzheimer Disease

Organs/Tissues associated with anti-LIPE antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >30 publications with LIPE and Blood
 Brain Antibodies  >28 publications with LIPE and Brain
 Adipose Tissue Antibodies  >15 publications with LIPE and Adipose Tissue
 Vascular Antibodies  >11 publications with LIPE and Vascular
 Kidney Antibodies  >5 publications with LIPE and Kidney
 Placenta Antibodies  >4 publications with LIPE and Placenta
 Embryonic Tissue Antibodies  >4 publications with LIPE and Embryonic Tissue
 Adrenal Gland Antibodies  >3 publications with LIPE and Adrenal Gland
 Testis Antibodies  >3 publications with LIPE and Testis
 Lung Antibodies  >3 publications with LIPE and Lung
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