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p73a Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610265

MBS610265 is an antibody targeting p73a. Also known as Anti - p73a (p73 alpha). Reactive in Human, Monkey. Host: RabbitSource: Human. More details

$980 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p73a, Polyclonal Antibody
Full Product Name
Anti - p73a (p73 alpha)
Product Gene Name
Product Synonym Names
p73a (p73 alpha)
Clonality
Polyclonal
Isotype
IgG
Host
Host: Rabbit
Source: Human
Species Reactivity
Human, Monkey
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Purity/Purification
Affinity purified over the control-peptide Sepharose
Concentration
1mg/ml (lot specific)
Specificity
The 20 AA human immunogenic peptide sequence has no significant homology with p53. It is conserved in monkey (100%), mouse (90%) and non-mammalian Barbus barbus.. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 20 amino acid peptide sequence mapping near the C-terminus of human p73a (1) was used to generate anti-human p73a. The peptide was coupled with KLH (carrier protein)
Applications Tested/Suitable for anti-p73a antibody
ELISA, Western Blot
Application Notes for anti-p73a antibody
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.

Product References and Citations for anti-p73a antibody

• :1. Kaghad, M et al (1997) Cell 90, 809-819;
• 2. Oren, M (1997) Cell 90, 829-832; Herranz M et al (1999) Cancer Res. 59, 2068-2071; Yang A et al (2000) Nature 404, 99-103;

Other Notes

Small volumes of anti-p73a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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