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HAKAI Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610267

Hakai was recently characterized as an E3 ubiquitin ligase related to the molecule c-Cbl1. c-Cbl is responsible for ubiquitinating the epithelial growth factor receptor (EGFR) and pro cadherins. Hakai is a novel E-cadherin binding protein, an E3 ligase which… More details

$715 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hakai, Polyclonal Antibody
Full Product Name
Hakai
Product Gene Name
Product Synonym Names
Anti -Hakai
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Canine
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity/Purification
Purified
Purified by epitope affinity chromatography.
Specificity
Recognizes canine Hakai. On Western blots, it identifies a single band at ~70kD, as well as a band at ~75-80kD that likely represents the ubiquitinated form of the Hakai protein. Reactivity has been confirmed with MDCK cell lysates. Species sequence homology: Mouse and human.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
32,559 Da
Chromosome Location
Chromosome: 2L; NT_033779.4 (19580612..19582474, complement). Location: 37F2-37F2
3D Structure
ModBase 3D Structure for Q9VIT1
Immunogen
Synthetic peptide derived from the C-terminal region of the human Hakai protein.
Positive Control
MDCK cell lysates

Product Categories/Family for anti-Hakai antibody

Applications Tested/Suitable for anti-Hakai antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Hakai antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml

Other Notes

Small volumes of anti-Hakai antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hakai purchase

MBS610267 is an antibody targeting Hakai. Reactive in Canine. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Hakai. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hakai, isoform F
NCBI Official Symbol
Hakai
NCBI Official Synonym Symbols
CG10263; DmelCG10263; hakai
UniProt Protein Name
Hakai, isoform A
UniProt Gene Name
Hakai
UniProt Entry Name
Q9VIT1_DROME
NCBI Protein Information
CG10263 gene product from transcript CG10263-RE; CG10263-PA; CG10263-PC; CG10263-PE; CG10263-PF; Hakai-PA; Hakai-PC; Hakai-PE; Hakai-PF; hakai

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-Hakai antibody

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