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Sprouty 4 (SPRY4) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610307

MBS610307 is an antibody targeting SPRY4. Also known as Sprouty 4 (Sprouty-4, Sprouty Homolog 4, Spry 4, SPRY4, SPRY-4). Reactive in Bovine, Canine, Human, Mouse, Rat. Host: Rabbit. More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Sprouty 4 (SPRY4), Polyclonal Antibody
Full Product Name
Sprouty 4 (Sprouty-4, Sprouty Homolog 4, Spry 4, SPRY4, SPRY-4)
Product Gene Name
Product Synonym Names
Anti -Sprouty 4 (Sprouty-4, Sprouty Homolog 4, Spry 4, SPRY4, SPRY-4)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Bovine, Canine, Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes Human Sprouty (Drosophila) Homolog 4 (SPRY4). Species Crossreactivity: Bovine, Dog, Human, Mouse, Rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
8,771 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (141689992..141704620, complement). Location: 5q31.3
3D Structure
ModBase 3D Structure for Q9BXY1
Immunogen
Synthetic peptide, KLH-conjugated. Epitope: Amino acids 306-322 of Human Sprouty-4 protein.

Product Categories/Family for anti-SPRY4 antibody

Applications Tested/Suitable for anti-SPRY4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SPRY4 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry: 2.5ug/ml
Western Blot: 1:100-1:1000
ELISA: 1:3000-1:12,000

Immunohistochemistry (IHC) of anti-SPRY4 antibody

SPRY4 (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human heart, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.
Rabbit Anti-SPRY4 Polyclonal Antibody – Cat# MBS610307

Other Notes

Small volumes of anti-SPRY4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPRY4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
sprouty 4
NCBI Official Synonym Full Names
sprouty homolog 4 (Drosophila)
NCBI Official Symbol
SPRY4
NCBI Official Synonym Symbols
SPRY4
UniProt Protein Name
Parathyroid hormone
Protein Family
UniProt Entry Name
Q9BXY1_HUMAN
NCBI Protein Information
protein sprouty homolog 4; spry-4; OTTHUMP00000160069; OTTHUMP00000222779

NCBI Summary for SPRY4

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).[supplied by OMIM]

UniProt Comments for SPRY4

SPRY4: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Belongs to the sprouty family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: Golgi apparatus; focal adhesion; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of MAP kinase activity; multicellular organismal development

Disease: Hypogonadotropic Hypogonadism 17 With Or Without Anosmia
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPRY4 antibody

Pathways associated with anti-SPRY4 antibody

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