Agmatine Antibody – Rabbit Polyclonal

Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Dilution: Immunohistochemistry (paraffin): 1:100 for 0.1-2.5% glutaraldehyde fixed tissues. Optimal fixation: 0.1-2.5% glutaraldehyde, 1% formalin. Minimum glutaraldehyde: 0.1%; works on paraffin embedded tissue (fixed with glutaraldehyde) but preferably epoxy embedded specifically developed for post-embedding protocols. Living, isolated goldfish retinas were maintained for 10 minutes in an oxygenated physiological solution that preserves normal neuronal activity under constant lighting. The medium also contained 10mM agmatine. After the incubation period retinas were fixed in a conventional 2.5% glutaraldehyde buffer and processed for immunohistochemistry using 250nm sections probed with a gold-labeled secondary antibody. The treated retinas were exposed to 125M kainate during the incubation. Kainate opens both AMPA and kainate selective ionotropic channels through which agmatine can enter cells.
Immunocytochemistry: On cells with glutamate-gated ion channels.