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ddit4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610486

REDD1 (REgulated in Development and DNA damage responses) expression is induced by hypoxia, cell stress, apoptosis and DNA damage. REDD1 is a transcriptional target of p53 and p63 following DNA damage, and links p63 to Ros (1). REDD1 functions as a negative… More details

$760 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
REDD1 (ddit4), Polyclonal Antibody
Full Product Name
REDD1 (Regulated in Development and DNA damage responses)
Product Gene Name
Product Synonym Names
Anti -REDD1 (Regulated in Development and DNA damage responses)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity/Purification
Purified by peptide affinity chromatography.
Specificity
Detects trans-fected levels of REDD1 protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
23,976 Da
Chromosome Location
Chromosome: 12; NC_007123.5 (49633414..49635564). Location: chromosome: 12
3D Structure
ModBase 3D Structure for Q7T346
Immunogen
Synthetic peptide (KLH-coupled) surrounding Asn60 of human REDD1.

Product Categories/Family for anti-ddit4 antibody

Applications Tested/Suitable for anti-ddit4 antibody
Western Blot (WB)
Application Notes for anti-ddit4 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000

Western Blot (WB) of anti-ddit4 antibody

Western blot analysis of extracts from COS-7 cells, transfected with HA-REDD1 or untransfected (mock), using MBS610486.
Rabbit Anti-ddit4 Polyclonal Antibody – Cat# MBS610486

Other Notes

Small volumes of anti-ddit4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ddit4 purchase

MBS610486 is an antibody targeting ddit4. Also known as REDD1 (Regulated in Development and DNA damage responses). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ddit4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
regulated in development and DNA damage response 1
NCBI Official Synonym Full Names
DNA-damage-inducible transcript 4
NCBI Official Symbol
ddit4
NCBI Official Synonym Symbols
redd1; rtp801; sb:cb821; zgc:64114
UniProt Protein Name
DNA damage-inducible transcript 4 protein
UniProt Gene Name
ddit4
UniProt Entry Name
DDIT4_DANRE
NCBI Protein Information
DNA damage-inducible transcript 4 protein; DNA-damage-inducible transcript 4 protein; regulated in development and DNA damage response 1

UniProt Comments for ddit4

Function: Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves ddit4/redd1, akt1, the tsc1-tsc2 complex and the GTPase rheb. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage, via its effect on mTORC1 activity. Plays a role in neuronal differentiation, neuron migration during embryonic brain development and in neuronal cell death

By similarity.

Subcellular location: Cytoplasm

By similarity. Mitochondrion

By similarity.

Sequence similarities: Belongs to the DDIT4 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ddit4 antibody

Pathways associated with anti-ddit4 antibody

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