Rabbit Anti-ATG10 Polyclonal Antibody – Cat# MBS610643

Confocal microscopy on immunofluorescently detected APG10 in cytospin-isolated human white blood cells using MBS610643at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.

ATG10 Antibody – Rabbit Polyclonal

Name: ATG10 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS610643
Price: 940 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610643

MBS610643 is an antibody targeting ATG10. Also known as Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10). Reactive in Human. Host: Rabbit. More details

$940 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Apg10 (ATG10), Polyclonal Antibody

Full Product Name

Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10)

Product Gene Name

anti-ATG10 antibody

Product Synonym Names

Anti -Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10)

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

Purity/Purification

Serum
Serum

Specificity

Recognizes human APG10. Species Crossreactivity: human

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

25,279 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (81267844..81551213). Location: 5q14.1

OMIM

610800

3D Structure

ModBase 3D Structure for Q9H0Y0

Immunogen

Synthetic peptide corresponding to the C-terminal of human APG10 protein

Product Categories/Family for anti-ATG10 antibody

Antibodies; Abs to Autophagy

Applications Tested/Suitable for anti-ATG10 antibody

Immunohistochemistry (IHC), Immunofluorescence (IF)

Application Notes for anti-ATG10 antibody

Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry.
Dilution: Immuhistochemistry (IF): 1:100-1:1000

Other Notes

Small volumes of anti-ATG10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG10. It may not necessarily be applicable to this product.

NCBI GI #

196162717

NCBI GeneID

83734

NCBI Accession #

NP_001124500.1

UniProt Primary Accession #

Q9H0Y0

UniProt Secondary Accession #

Q6PIX1; Q9H842; B2RE09

UniProt Related Accession #

Q9H0Y0

GenBank Nucleotide #

NM_001131028.1

NCBI Official Full Name

ubiquitin-like-conjugating enzyme ATG10

NCBI Official Synonym Full Names

ATG10 autophagy related 10 homolog (S. cerevisiae)

NCBI Official Symbol

ATG10

NCBI Official Synonym Symbols

APG10; APG10L; pp12616; FLJ13954; DKFZp586I0418

UniProt Protein Name

Ubiquitin-like-conjugating enzyme ATG10

UniProt Synonym Protein Names

Autophagy-related protein 10

Protein Family

Ubiquitin-like-conjugating enzyme

UniProt Gene Name

ATG10

UniProt Entry Name

ATG10_HUMAN

NCBI Protein Information

ubiquitin-like-conjugating enzyme ATG10; OTTHUMP00000147582; autophagy-related protein 10

NCBI Summary for ATG10

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM]

UniProt Comments for ATG10

ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytoplasm; intracellular

Molecular Function: APG12 conjugating enzyme activity; ligase activity

Biological Process: protein transport; protein modification by small protein conjugation; protein amino acid lipidation; positive regulation of protein modification process; autophagy

Research Articles on ATG10

1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG10 antibody

Reference Product	PubMed Publications
ATG5 antibody	>19 publications with ATG10 and ATG5
ATG12 antibody	>16 publications with ATG10 and ATG12
ATG7 antibody	>8 publications with ATG10 and ATG7

Pathways associated with anti-ATG10 antibody

Products by Pathway	Pathway Diagram
Senescence And Autophagy Pathway antibodies	Senescence And Autophagy Pathway Diagram

Diseases associated with anti-ATG10 antibody

Disease Name	Pubmed Publications
Leukemia, Myeloid, Acute Antibodies	>1 publications with ATG10 and Leukemia, Myeloid, Acute
Neoplasms Antibodies	>1 publications with ATG10 and Neoplasms
Myelodysplastic Syndromes Antibodies	>1 publications with ATG10 and Myelodysplastic Syndromes

Organs/Tissues associated with anti-ATG10 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with ATG10 and Brain
Bone Antibodies	>1 publications with ATG10 and Bone