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ROBO4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610706

Roundabouts (ROBO) are cell-surface receptors that mediate repulsive signaling mechanisms at the central nervous system midline. However, ROBOs may also mediate attraction mechanisms in the context of vascular development. ROBO4 is a novel roundabout protein… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Robo4, Polyclonal Antibody
Full Product Name
Robo4 (Roundabout Homolog 4, Roundabout Homolog 4 Magic Roundabout, Roundabout Homolog 4 Precursor, FLJ20798, Magic Roundabout, MRB, MGC133352, MGC133353)
Product Gene Name
Product Synonym Names
Anti -Robo4 (Roundabout Homolog 4, Roundabout Homolog 4 Magic Roundabout, Roundabout Homolog 4 Precursor, FLJ20798, Magic Roundabout, MRB, MGC133352, MGC133353)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Tris-citrate/phosphate [pH 7-8]
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
This antibody recognizes the 85kD and 107kD isoforms of the ROBO4 human protein. Species Crossreactivity: This antibody reacts with human protein. Other species have not been tested.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
107,457 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (124754114..124767831, complement). Location: 11q24.2
OMIM
607528
3D Structure
ModBase 3D Structure for Q8WZ75
Immunogen
A synthetic peptide made to a C-terminal region of the human protein (within residues 900-1007). [Swiss-Prot# Q8WZ75]. Homology: 100% homology with the human protein. There is no homology with mouse or rat proteins.
Positive Control
HUVEC lysate

Product Categories/Family for anti-ROBO4 antibody

Applications Tested/Suitable for anti-ROBO4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ROBO4 antibody
Suitable for use in Western Blot and Immunohistochemistry.

Other Notes

Small volumes of anti-ROBO4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ROBO4 purchase

MBS610706 is an antibody targeting ROBO4. Also known as Robo4 (Roundabout Homolog 4, Roundabout Homolog 4 Magic Roundabout, Roundabout Homolog 4 Precursor, FLJ20798, Magic Roundabout, MRB, MGC133352, MGC133353). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ROBO4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
roundabout homolog 4
NCBI Official Synonym Full Names
roundabout homolog 4, magic roundabout (Drosophila)
NCBI Official Symbol
ROBO4
NCBI Official Synonym Symbols
MRB; ECSM4; FLJ20798; MGC133352; MGC133353
UniProt Protein Name
Roundabout homolog 4
UniProt Synonym Protein Names
Magic roundabout
Protein Family
UniProt Gene Name
ROBO4
UniProt Entry Name
ROBO4_HUMAN
NCBI Protein Information
roundabout homolog 4; OTTHUMP00000231537; OTTHUMP00000231538

UniProt Comments for ROBO4

ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: receptor activity

Biological Process: angiogenesis; cell differentiation; regulation of cell migration; negative regulation of cell migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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