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Endomucin (EMCN) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610782

Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80-120kD glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Endomucin (EMCN), Polyclonal Antibody
Full Product Name
Endomucin
Product Gene Name
Product Synonym Names
Anti -Endomucin
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Mouse
Form/Format
Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Endomucin.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
27,452 Da
Chromosome Location
Chromosome: 4; NC_000004.11 (101316498..101439250, complement). Location: 4q24
3D Structure
ModBase 3D Structure for Q4W5J1
Immunogen
E. coli-derived, recombinant mouse Endomucin (aa 21 - 90).
Applications Tested/Suitable for anti-EMCN antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-EMCN antibody
Suitable for use in Flow cytometry, Direct ELISA, Western Blot and Immunocytochemistry.
Dilution: Flow Cytometry: 25ug/mL use 10ul to label 10e6 cells
Direct ELISA: 0.5-1ug/mL detection limit for rmEndomucin ~ 0.3ng/well.
Western Blot: 0.1-0.2ug/mL detection limit for rmEndomucin ~ 1ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/mL

Other Notes

Small volumes of anti-EMCN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EMCN purchase

MBS610782 is an antibody targeting EMCN. Also known as Endomucin. Reactive in Mouse. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for EMCN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endomucin isoform 2
NCBI Official Synonym Full Names
endomucin
NCBI Official Symbol
EMCN
NCBI Official Synonym Symbols
EMCN2; MUC14
UniProt Protein Name
Endomucin, isoform CRA_a
Protein Family
UniProt Gene Name
EMCN
UniProt Entry Name
Q4W5J1_HUMAN
NCBI Protein Information
endomucin; MUC-14; mucin-14; endomucin-2; OTTHUMP00000161682; OTTHUMP00000219639; OTTHUMP00000219640; gastric cancer antigen Ga34

NCBI Summary for EMCN

EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM]

UniProt Comments for EMCN

EMCN: Endothelial sialomucin, also called endomucin or mucin- like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: plasma membrane; integral to membrane; extracellular region
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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