Rabbit Anti-IRE1 Polyclonal Antibody – Cat# MBS610790

Western blot analysis of IRE1p in Raji cell lysate with IRE1p antibody at (A) 1ug/ml and (B) 2ug/ml.

Ire1p (IRE1) Antibody – Rabbit Polyclonal

Name: IRE1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS610790
Price: 850 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610790

Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94 (1,2). These proteins are normally bound to ER transmembrane proteins such… More details

$850 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IRE1p, Polyclonal Antibody

Full Product Name

IRE1p, NT (Inositol-Requiring Protein 1, IRE1a, Endoplasmic Reticulum-to-Nucleus Signaling 1, ERN1)

Product Gene Name

anti-IRE1 antibody

Product Synonym Names

Anti -IRE1p, NT (Inositol-Requiring Protein 1, IRE1a, Endoplasmic Reticulum-to-Nucleus Signaling 1, ERN1)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS pH 7.2, 0.02% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human IRE1p.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

126,976 Da

Chromosome Location

Chromosome: VIII; NC_001140.6 (258244..261591, complement)

3D Structure

ModBase 3D Structure for P32361

Immunogen

15 amino acid peptide from near the amino terminus of human IRE1p (Genbank accession No. O75460).

Product Categories/Family for anti-IRE1 antibody

Antibodies; Abs to Protein Kinases

Applications Tested/Suitable for anti-IRE1 antibody

Western Blot (WB)

Application Notes for anti-IRE1 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 1-2ug/ml
Optimal dilutions to be determined by researcher.

Other Notes

Small volumes of anti-IRE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRE1 purchase

MBS610790 is an antibody targeting IRE1. Also known as IRE1p, NT (Inositol-Requiring Protein 1, IRE1a, Endoplasmic Reticulum-to-Nucleus Signaling 1, ERN1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IRE1. It may not necessarily be applicable to this product.

NCBI GI #

6321870

NCBI GeneID

856478

NCBI Accession #

NP_011946.1

UniProt Primary Accession #

P32361

UniProt Secondary Accession #

D3DL29

UniProt Related Accession #

P32361

GenBank Nucleotide #

NM_001179209.1

NCBI Official Full Name

Ire1p

NCBI Official Symbol

IRE1

NCBI Official Synonym Symbols

ERN1

UniProt Protein Name

Serine/threonine-protein kinase/endoribonuclease IRE1

UniProt Synonym Protein Names

Endoplasmic reticulum-to-nucleus signaling 1

Protein Family

Serine/threonine-protein kinase/endoribonuclease

UniProt Gene Name

IRE1

UniProt Synonym Gene Names

ERN1

UniProt Entry Name

IRE1_YEAST

NCBI Protein Information

Ire1p

UniProt Comments for IRE1

Function: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices HAC1 precursor mRNA to produce the mature form which then induces transcription of UPR target genes. Ref.5 Ref.6 Ref.7

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ref.5

Subunit structure: Homodimer; in response to the accumulation of unfolded proteins. Ref.5 Ref.6

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Post-translational modification: Autophosphorylated mainly on serine residues. Ref.6

Miscellaneous: Present with 259 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 KEN domain.Contains 1 protein kinase domain.

Sequence caution: The sequence AAB68894.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on IRE1

1. Flavonol activation defines an unanticipated ligand-binding site in the kinase-RNase domain of IRE1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IRE1 antibody

Reference Product	PubMed Publications
SPT20 antibody	>2 publications with IRE1 and SPT20
ADA2 antibody	>2 publications with IRE1 and ADA2

Pathways associated with anti-IRE1 antibody

Products by Pathway	Pathway Diagram
Activation Of Chaperones By IRE1alpha Pathway antibodies	Activation Of Chaperones By IRE1alpha Pathway Diagram
Diabetes Pathways antibodies	Diabetes Pathways Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Unfolded Protein Response Pathway antibodies	Unfolded Protein Response Pathway Diagram