Rabbit Anti-RPS6KA3 Polyclonal Antibody – Cat# MBS610850

Lysates from A431 cells unstimulated (1) or stimulated with 100 ng/mL PMA . Prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5).

RSK1, RSK2 (RPS6KA3) Antibody – Rabbit Polyclonal

Name: RPS6KA3 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS610850
Price: 1010 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS610850

RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c) and RSK4. RSK proteins are substrates… More details

$1010 / 10 Blots +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RSK1, RSK2 (Rps6ka3), Polyclonal Antibody

Full Product Name

RSK1, RSK2, phosphorylated (Ser221/Ser227) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ri

Product Gene Name

anti-Rps6ka3 antibody

Product Synonym Names

Anti -RSK1, RSK2, phosphorylated (Ser221/Ser227) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ri

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Chicken, Human, Mouse

Form/Format

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.

Purity/Purification

Affinity Purified
Purified by affinity chromatography.

Specificity

Recognizes human Rsk1, Rsk2, phosphorylated, Ser221, Ser227.. Species Crossreactivity

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

83,694 Da

Chromosome Location

Chromosome: X; NC_000086.7 (159256120..159363485). Location: X F4; X 73.27 cM

3D Structure

ModBase 3D Structure for P18654

Immunogen

Synthetic phosphopeptide derived from a region of human RSK1 that contains serine 221.

Positive Control

A431 cells treated with 100ng/mL PMA for 30 minutes at 37ºC; HEK293E cells transfected with human RSK1, chicken RSK1, mouse RSK2, or human RSK3.

Product Categories/Family for anti-Rps6ka3 antibody

Antibodies; Abs to Protein Kinases; Ribosomal

Applications Tested/Suitable for anti-Rps6ka3 antibody

Western Blot (WB)

Application Notes for anti-Rps6ka3 antibody

Suitable for use in Western Blot. Other applications not tested
Dilution: Western Blot: 1:1000
Optimal dilution determined by the researcher.

Other Notes

Small volumes of anti-Rps6ka3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rps6ka3 purchase

MBS610850 is an antibody targeting Rps6ka3. Also known as RSK1, RSK2, phosphorylated (Ser221/Ser227) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ri. Reactive in Chicken, Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Rps6ka3. It may not necessarily be applicable to this product.

NCBI GI #

22507357

NCBI GeneID

110651

NCBI Accession #

NP_683747.1

UniProt Primary Accession #

P18654

UniProt Secondary Accession #

Q03140; Q8K3J8; B1AXN4

UniProt Related Accession #

P18654

GenBank Nucleotide #

NM_148945.1

NCBI Official Full Name

ribosomal protein S6 kinase alpha-3

NCBI Official Synonym Full Names

ribosomal protein S6 kinase polypeptide 3

NCBI Official Symbol

Rps6ka3

NCBI Official Synonym Symbols

Rsk2; MPK-9; pp90RSK2; S6K-alpha3

UniProt Protein Name

Ribosomal protein S6 kinase alpha-3

UniProt Synonym Protein Names

90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; p90RSK3; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; Ribosomal S6 kinase 2; RSK-2; pp90RSK2

Protein Family

Ribosomal protein S6 kinase

UniProt Gene Name

Rps6ka3

UniProt Synonym Gene Names

Mapkapk1b; Rps6ka-rs1; Rsk2; S6K-alpha-3; p90-RSK 3; p90RSK3; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2

UniProt Entry Name

KS6A3_MOUSE

NCBI Protein Information

ribosomal protein S6 kinase alpha-3; RSK-2; p90RSK3; p90-RSK 3; MAPKAPK-1b; S6K-alpha-3; MAPKAP kinase 1b; ribosomal S6 kinase 2; ribosomal protein S6 kinase 2; MAPK-activated protein kinase 1b; 90 kDa ribosomal protein S6 kinase 3; MAP kinase-activated protein kinase 1b

UniProt Comments for Rps6ka3

RSK2: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; RSK family; RSK subfamily

Cellular Component: cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; caspase inhibitor activity; transferase activity, transferring phosphorus-containing groups; magnesium ion binding; nucleotide binding; kinase activity; ribosomal protein S6 kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: regulation of translation; cellular response to stress; toll-like receptor signaling pathway; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; cell cycle; phosphorylation; protein amino acid phosphorylation

Research Articles on Rps6ka3

1. Pin1 plays a critical role in tumorigenesis plausibly via physical interaction with RSK2 and reciprocal phosphorylation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Rps6ka3 antibody

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies	Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
CREB Phosphorylation Pathway antibodies	CREB Phosphorylation Pathway Diagram
CREB Phosphorylation Through The Activation Of Ras Pathway antibodies	CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
Cytoplasmic Ribosomal Proteins Pathway antibodies	Cytoplasmic Ribosomal Proteins Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
ERK/MAPK Targets Pathway antibodies	ERK/MAPK Targets Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram

Diseases associated with anti-Rps6ka3 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>27 publications with Rps6ka3 and Nervous System Diseases
Coffin-Lowry Syndrome Antibodies	>24 publications with Rps6ka3 and Coffin-Lowry Syndrome
Disease Models, Animal Antibodies	>5 publications with Rps6ka3 and Disease Models, Animal
Congenital Abnormalities Antibodies	>5 publications with Rps6ka3 and Congenital Abnormalities
Brain Diseases Antibodies	>2 publications with Rps6ka3 and Brain Diseases
Nervous System Malformations Antibodies	>2 publications with Rps6ka3 and Nervous System Malformations
Carcinoma, Hepatocellular Antibodies	>2 publications with Rps6ka3 and Carcinoma, Hepatocellular
Neural Tube Defects Antibodies	>1 publications with Rps6ka3 and Neural Tube Defects
Liver Diseases Antibodies	>1 publications with Rps6ka3 and Liver Diseases
Learning Disorders Antibodies	>1 publications with Rps6ka3 and Learning Disorders

Organs/Tissues associated with anti-Rps6ka3 antibody

Organ/Tissue Name	Pubmed Publications
Other Antibodies	>10 publications with Rps6ka3 and Other
Brain Antibodies	>9 publications with Rps6ka3 and Brain
Liver Antibodies	>2 publications with Rps6ka3 and Liver
Embryonic Tissue Antibodies	>2 publications with Rps6ka3 and Embryonic Tissue
Connective Tissue Antibodies	>1 publications with Rps6ka3 and Connective Tissue
Heart Antibodies	>1 publications with Rps6ka3 and Heart
Testis Antibodies	>1 publications with Rps6ka3 and Testis
Ovary Antibodies	>1 publications with Rps6ka3 and Ovary
Bone Marrow Antibodies	>1 publications with Rps6ka3 and Bone Marrow