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P4HTM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS611027

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those… More details

$955 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PHD4 (P4HTM), Polyclonal Antibody
Full Product Name
PHD4 (PH4, PH-4, Prolyl Hydroxlase Domain-containing 4, Prolyl Hydroxylase 4, HIF Prolyl Hydroxylase 4, HIFPH4, Hypoxia-inducible Factor Prolyl 4-hydroxylase, HIF Prolyl 4-hydroxylase, FLJ20262, Hypoxia Inducible Factor Prolyl Hydroxylase, P4H with Transm
Product Gene Name
anti-P4HTM antibody
Product Synonym Names
Anti -PHD4 (PH4, PH-4, Prolyl Hydroxlase Domain-containing 4, Prolyl Hydroxylase 4, HIF Prolyl Hydroxylase 4, HIFPH4, Hypoxia-inducible Factor Prolyl 4-hydroxylase, HIF Prolyl 4-hydroxylase, FLJ20262, Hypoxia Inducible Factor Prolyl Hydroxylase, P4H with Transm
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Tris-citrate/phosphate [pH 7-8]
Purity/Purification
Affinity Purified
Purified by peptide immunoaffinity chromatography.
Specificity
This antibody is specific for prolyl hydroxylase 4. Species Crossreactivity: Human. Does not appear to work in mouse. Other species have not been tested.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
56,661 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (49027304..49044582). Location: 3p21.31; 3p21.3
OMIM
614584
3D Structure
ModBase 3D Structure for Q9NXG6
Immunogen
A synthetic peptide made to a portion of the C-terminus of the human prolyl hydroxylase domain-containing 4 encoded within exon 4. [LocusLink 54681].
Positive Control
HeLa and human glioblastoma cell lysates

Product Categories/Family for anti-P4HTM antibody

Antibodies; Abs to Hypoxia
Applications Tested/Suitable for anti-P4HTM antibody
Western Blot (WB)
Application Notes for anti-P4HTM antibody
Suitable for use in Western Blot.

Other Notes

Small volumes of anti-P4HTM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for P4HTM purchase

MBS611027 is an antibody targeting P4HTM. Also known as PHD4 (PH4, PH-4, Prolyl Hydroxlase Domain-containing 4, Prolyl Hydroxylase 4, HIF Prolyl Hydroxylase 4, HIFPH4, Hypoxia-inducible Factor Prolyl 4-hydroxylase, HIF Prolyl 4-hydroxylase, FLJ20262, Hypoxia Inducible Factor Prolyl Hydroxylase, P4H with Transm. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for P4HTM. It may not necessarily be applicable to this product.

NCBI GI #
530372670
NCBI GeneID
54681
NCBI Accession #
XP_005265292.1
UniProt Primary Accession #
Q9NXG6
UniProt Secondary Accession #
Q6PAG6; Q8TCJ9; Q8WV55; Q96F22; Q9BW77
UniProt Related Accession #
Q9NXG6
GenBank Nucleotide #
XM_005265235.1
NCBI Official Full Name
transmembrane prolyl 4-hydroxylase isoform X1
NCBI Official Synonym Full Names
prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
NCBI Official Symbol
P4HTM
NCBI Official Synonym Symbols
PH4; PH-4; PHD4; EGLN4; HIFPH4; P4H-TM
UniProt Protein Name
Transmembrane prolyl 4-hydroxylase
UniProt Synonym Protein Names
Hypoxia-inducible factor prolyl hydroxylase 4
Protein Family
Transmembrane prolyl 4-hydroxylase
UniProt Gene Name
P4HTM
UniProt Synonym Gene Names
PH4; P4H-TM; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4
UniProt Entry Name
P4HTM_HUMAN
NCBI Protein Information
transmembrane prolyl 4-hydroxylase; HPH-4; HIF-PH4; proline 4-hydroxylase; HIF-prolyl hydroxylase 4; P4H with transmembrane domain; Prolyl hydroxlase domain-containing 4; hypoxia inducible factor prolyl 4 hydroxylase; hypoxia-inducible factor prolyl 4-hydroxylase; hypoxia-inducible factor prolyl hydroxylase 4

NCBI Summary for P4HTM

The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for P4HTM

PH4: Catalyzes the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.14.11.-

Chromosomal Location of Human Ortholog: 3p21.31|3p21.3

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; calcium ion binding
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Research Articles on P4HTM

1. Results suggest that increased expression of prolyl hydroxylase (PH) might play an role in the physiology of uterine leiomyoma during the menstrual cycle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-P4HTM antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with P4HTM and Neoplasms

Organs/Tissues associated with anti-P4HTM antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with P4HTM and Muscle
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