• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Thyroid Iodide Transporter (TPO) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS611227

Iodide (I-) is an essential constituent of thyroid hormones. Thyroid epithelial cells for the biosynthesis of thyroid hormones actively transport it. At the cellular level, Iodide transport is mediated by intrinsic membrane Na+/I+ symporter (NIS/TIT). Rat… More details

$965 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thyroid Iodide Transporter (Tpo), Polyclonal Antibody
Full Product Name
Thyroid Iodide Transporter (TIT)
Product Gene Name
anti-Tpo antibody
Product Synonym Names
Anti -Thyroid Iodide Transporter (TIT)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat Thyroid Iodide Transporter. No significant sequence homology is seen with glucose transporter or other Na+-dependent transporters.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
101,460 Da
Chromosome Location
Chromosome: 6; NC_005105.3 (57702103..57770914, complement). Location: 6q16
3D Structure
ModBase 3D Structure for P14650
Immunogen
A 16 AA peptide sequence, near the C-terminal, cytoplasmic domain of rat TIT (1) was synthesized, coupled to KLH

Product Categories/Family for anti-Tpo antibody

Antibodies; Abs to Transport Proteins
Applications Tested/Suitable for anti-Tpo antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-Tpo antibody
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Dilution: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1-10ug/ml (ECL); A band of 95-110kD has been detected in brush border membrane (2).
Immunofluorescence: 2-20ug/ml
Immunohistochemistry: 2-20ug/ml

Other Notes

Small volumes of anti-Tpo antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Tpo purchase

MBS611227 is an antibody targeting Tpo. Also known as Thyroid Iodide Transporter (TIT). Reactive in Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Tpo. It may not necessarily be applicable to this product.

NCBI GI #
402766537
NCBI GeneID
54314
NCBI Accession #
NP_062226.2
UniProt Primary Accession #
P14650
UniProt Related Accession #
P14650
GenBank Nucleotide #
NM_019353.2
NCBI Official Full Name
thyroid peroxidase
NCBI Official Synonym Full Names
thyroid peroxidase
NCBI Official Symbol
Tpo
UniProt Protein Name
Thyroid peroxidase
Protein Family
Thyroid peroxidase
UniProt Gene Name
Tpo
UniProt Synonym Gene Names
TPO
UniProt Entry Name
PERT_RAT
NCBI Protein Information
thyroid peroxidase

NCBI Summary for Tpo

membrane-bound glycoprotein; iodinates tyrosine residues in thyroglobulin to generate biologically active thyroid hormones T3 and T4 [RGD, Feb 2006]

UniProt Comments for Tpo

Function: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Catalytic activity: 2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.[Thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O.[Thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O.2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O.[Thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O.

Cofactor: Binds 1 calcium ion per heterodimer

By similarity.Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer

By similarity.

Pathway: Hormone biosynthesis; thyroid hormone biosynthesis.

Subunit structure: Interacts with DUOX1, DUOX2 and CYBA

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Post-translational modification: Heme is covalently bound through a H2O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface

By similarity.Cleaved in its N-terminal part

By similarity.

Sequence similarities: Belongs to the peroxidase family. XPO subfamily.Contains 1 EGF-like domain.Contains 1 Sushi (CCP/SCR) domain.
Show more

Research Articles on Tpo

1. Thyroid peroxidase gene expression is induced by lipopolysaccharide involving nuclear factor (NF)-kappaB p65 subunit phosphorylation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Tpo antibody

 Reference Product  PubMed Publications
 Tyr antibody  >9 publications with Tpo and Tyr
 Tat antibody  >4 publications with Tpo and Tat
 Iyd antibody  >3 publications with Tpo and Iyd
 Tshb antibody  >2 publications with Tpo and Tshb

Pathways associated with anti-Tpo antibody

 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Organs/Tissues associated with anti-Tpo antibody

 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >60 publications with Tpo and Mixed
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000