Rabbit Anti-MAP2K4 Polyclonal Antibody – Cat# MBS611593

Western blot analysis of extracts from HeLa and C6 cells, untreated or UV-treated, using MBS611593 or J2475-05B (lower).

MAP2K4 Antibody – Rabbit Polyclonal

Name: MAP2K4 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS611593
Price: 830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS611593

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through phosphorylation of serine and… More details

$830 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SEK1 (Map2k4), Polyclonal Antibody

Full Product Name

SEK1, phosphorylated (Ser257,Thr261) (MKK4, SAPK, Erk Kinase, MKK4, Jun Kinase Kinase, JNKK)

Product Gene Name

anti-Map2k4 antibody

Product Synonym Names

Anti -SEK1, phosphorylated (Ser257,Thr261) (MKK4, SAPK, Erk Kinase, MKK4, Jun Kinase Kinase, JNKK)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Drosophila, Human, Mouse, Rat

Form/Format

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol

Purity/Purification

Affinity Purified
Purified by Protein A and peptide affinity chromatography.

Specificity

Recognizes endogenous levels of human SEK1/MKK4 only when phosphorylated at serine 257 and threonine 261. Does not crossreact with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3. Species Crossreactivity

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

44,114 Da

Chromosome Location

Chromosome: 11; NC_000077.5 (65501746..65601799, complement). Location: 11 B3; 11 33.0 cM

3D Structure

ModBase 3D Structure for P47809

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser257/Thr261 of human SEK1 (KLH).

Product Categories/Family for anti-Map2k4 antibody

Antibodies; Abs to Protein Kinases

Applications Tested/Suitable for anti-Map2k4 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-Map2k4 antibody

Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000

Other Notes

Small volumes of anti-Map2k4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Map2k4 purchase

MBS611593 is an antibody targeting Map2k4. Also known as SEK1, phosphorylated (Ser257,Thr261) (MKK4, SAPK, Erk Kinase, MKK4, Jun Kinase Kinase, JNKK). Reactive in Drosophila, Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Map2k4. It may not necessarily be applicable to this product.

NCBI GI #

1754531

NCBI GeneID

26398

UniProt Primary Accession #

P47809

UniProt Related Accession #

P47809; Q543X6; Q8K2U0

NCBI Official Full Name

SEK1

NCBI Official Synonym Full Names

mitogen-activated protein kinase kinase 4

NCBI Official Symbol

Map2k4

NCBI Official Synonym Symbols

MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4

UniProt Protein Name

Dual specificity mitogen-activated protein kinase kinase 4

UniProt Synonym Protein Names

C-JUN N-terminal kinase kinase 1; JNK kinase 1; JNKK 1; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1

Protein Family

Dual specificity mitogen-activated protein kinase kinase

UniProt Gene Name

Map2k4

UniProt Synonym Gene Names

Jnkk1; Prkmk4; Sek1; Serk1

UniProt Entry Name

MP2K4_MOUSE

NCBI Protein Information

dual specificity mitogen-activated protein kinase kinase 4; MEK 4; JNKK 1; MAPKK 4; JNK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; SAPK/Erk/kinase 1; OTTMUSP00000006102; MAP kinase kinase 4; JNK-activating kinase 1; C-JUN N-terminal kinase kinase 1; mitogen activated protein kinase kinase 4, variant 1

UniProt Comments for Map2k4

MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.

Protein type: Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; EC 2.7.12.2; STE group; STE7 family

Cellular Component: dendrite cytoplasm; axon; cytoplasm; perikaryon; intracellular; cytosol; nucleus

Molecular Function: transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of neuron apoptosis; MAPKKK cascade; JNK cascade; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; phosphorylation; positive regulation of DNA replication; activation of JNK activity; positive regulation of nitric-oxide synthase biosynthetic process

Research Articles on Map2k4

1. 7,3',4'-Trihydroxyisoflavone, a metabolite of the soy isoflavone daidzein, suppresses ultraviolet B-induced skin cancer by targeting Cot and MKK4

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Map2k4 antibody

Reference Product	PubMed Publications
Map3k1 antibody	>40 publications with Map2k4 and Map3k1

Pathways associated with anti-Map2k4 antibody

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
ErbB Signaling Pathway antibodies	ErbB Signaling Pathway Diagram
ErbB Signaling Pathway antibodies	ErbB Signaling Pathway Diagram
FAS Pathway And Stress Induction Of HSP Regulation antibodies	FAS Pathway And Stress Induction Of HSP Regulation Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram

Diseases associated with anti-Map2k4 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>72 publications with Map2k4 and Neoplasms
Carcinoma Antibodies	>22 publications with Map2k4 and Carcinoma
Urogenital Neoplasms Antibodies	>17 publications with Map2k4 and Urogenital Neoplasms
Neoplasm Metastasis Antibodies	>17 publications with Map2k4 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>15 publications with Map2k4 and Breast Neoplasms
Skin Diseases Antibodies	>14 publications with Map2k4 and Skin Diseases
Ovarian Neoplasms Antibodies	>11 publications with Map2k4 and Ovarian Neoplasms
Lung Neoplasms Antibodies	>11 publications with Map2k4 and Lung Neoplasms
Adenocarcinoma Antibodies	>11 publications with Map2k4 and Adenocarcinoma
Female Urogenital Diseases Antibodies	>10 publications with Map2k4 and Female Urogenital Diseases

Organs/Tissues associated with anti-Map2k4 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>21 publications with Map2k4 and Brain
Lung Antibodies	>15 publications with Map2k4 and Lung
Kidney Antibodies	>13 publications with Map2k4 and Kidney
Liver Antibodies	>12 publications with Map2k4 and Liver
Skin Antibodies	>9 publications with Map2k4 and Skin
Bone Antibodies	>5 publications with Map2k4 and Bone
Thymus Antibodies	>5 publications with Map2k4 and Thymus
Embryonic Tissue Antibodies	>5 publications with Map2k4 and Embryonic Tissue
Heart Antibodies	>4 publications with Map2k4 and Heart
Bone Marrow Antibodies	>3 publications with Map2k4 and Bone Marrow