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Paired Immunoglobulin-Like Type 2 Receptor-Ligand (CD99) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS611785

PILR-L (also known as CD99 and MIC2) is a 125 amino acid, 24-30 kD glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. PILR-L has been implicated in processes like homotypic… More details

$1090 / 0.1 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Paired Immunoglobulin-Like Type 2 Receptor-Ligand (Cd99), Polyclonal Antibody
Full Product Name
Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L, CD99, MIC2)
Product Gene Name
Product Synonym Names
Anti -Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L, CD99, MIC2)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Mouse
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Purity/Purification
Affinity Purified
Purified by mouse PILR-L affinity chromatography.
Specificity
Selected for its ability to recognize mouse PILR-L in direct ELISAs and Western Blots.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
16,783 Da
Chromosome Location
4; 4
3D Structure
ModBase 3D Structure for Q8VCN6
Immunogen
NS0-derived rmPILR-L (aa25-138)

Product Categories/Family for anti-Cd99 antibody

Applications Tested/Suitable for anti-Cd99 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Cd99 antibody
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 2 ng/lane under non-reducing and reducing conditions, respectively., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 0.1 ng/well. In this format, this antibody shows less than 1% crossreactivity with rmPILR- and rmPILR-., Optimal dilutions should be determined by each laboratory for each application.

Other Notes

Small volumes of anti-Cd99 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cd99 purchase

MBS611785 is an antibody targeting Cd99. Also known as Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L, CD99, MIC2). Reactive in Mouse. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for Cd99. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
paired immunoglobin-like type 2 receptor-ligand
NCBI Official Synonym Full Names
CD99 antigen
NCBI Official Symbol
Cd99
NCBI Official Synonym Symbols
D4; Pilr-l; pilr-1; 1110061M03Rik; 2410026K10Rik
UniProt Protein Name
CD99 antigen
UniProt Synonym Protein Names
Paired immunoglobin-like type 2 receptor-ligand
Protein Family
UniProt Gene Name
Cd99
UniProt Synonym Gene Names
Pilrl
UniProt Entry Name
CD99_MOUSE
NCBI Protein Information
CD99 antigen; paired immunoglobin-like type 2 receptor-ligand

UniProt Comments for Cd99

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane

Molecular Function: protein binding

Biological Process: myeloid dendritic cell activation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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