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P47 Phox (NCF1) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS611915

Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal… More details

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Product Overview

Online Datasheet in PDF
Product Name
p47 phox (NCF1), Polyclonal Antibody
Full Product Name
p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1, 47kD, chronic granulomatous disease, autosomal 1, Neutrophil cytosolic factor-1 (47kD)
Product Gene Name
anti-NCF1 antibody
Product Synonym Names
Anti -p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1, 47kD, chronic granulomatous disease, autosomal 1, Neutrophil cytosolic factor-1 (47kD)
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide before the addition of glycerol to 40%.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes Human NCF1/p47phox. Species Sequence Homology: Mouse, rat, rabbit, bovine.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
44,652 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (74188309..74203659). Location: 7q11.23
OMIM
233700
3D Structure
ModBase 3D Structure for P14598
Immunogen
Synthetic peptide CSESTKRKLASAV, corresponding to C Terminus of human p47 phox.

Product Categories/Family for anti-NCF1 antibody

Antibodies; Abs to Neutrophils
Applications Tested/Suitable for anti-NCF1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-NCF1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 0.2-2ug/ml~47kD band seen in U937 cell lysates.
Optimal dilution to be determined by researcher.

Chemiluminescence of anti-NCF1 antibody

Staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NCF1 Polyclonal Antibody – Cat# MBS611915

Chemiluminescence of anti-NCF1 antibody

Staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NCF1 Polyclonal Antibody, Chemiluminescence – Cat# MBS611915

Other Notes

Small volumes of anti-NCF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NCF1 purchase

MBS611915 is an antibody targeting NCF1. Also known as p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1, 47kD, chronic granulomatous disease, autosomal 1, Neutrophil cytosolic factor-1 (47kD). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for NCF1. It may not necessarily be applicable to this product.

NCBI GI #
2754713
NCBI GeneID
653361
UniProt Primary Accession #
P14598
UniProt Secondary Accession #
O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9
UniProt Related Accession #
P14598
NCBI Official Full Name
p47-phox
NCBI Official Synonym Full Names
neutrophil cytosolic factor 1
NCBI Official Symbol
NCF1
NCBI Official Synonym Symbols
NCF1A; NOXO2; p47phox; FLJ79451; SH3PXD1A
UniProt Protein Name
Neutrophil cytosol factor 1
UniProt Synonym Protein Names
47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
Protein Family
Neutrophil cytosol factor
UniProt Gene Name
NCF1
UniProt Synonym Gene Names
NOXO2; SH3PXD1A
UniProt Entry Name
NCF1_HUMAN
NCBI Protein Information
neutrophil cytosol factor 1; NCF-1; NCF-47K; p47-phox; nox organizer 2; OTTHUMP00000195569; nox-organizing protein 2; NADPH oxidase organizer 2; 47 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 1; SH3 and PX domain-containing protein 1A; 47 kDa autosomal chronic granulomatous disease protein; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)

NCBI Summary for NCF1

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq]

UniProt Comments for NCF1

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; extrinsic to membrane; cell soma; rough endoplasmic reticulum; dendrite; cytosol; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; superoxide-generating NADPH oxidase activity; phosphatidylinositol-3,4-bisphosphate binding; SH3 domain binding; phosphoinositide binding

Biological Process: respiratory burst during defense response; respiratory burst; interaction with host; apoptosis; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; leukotriene metabolic process; cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; negative regulation of smooth muscle contraction; innate immune response; cellular defense response; antigen processing and presentation of exogenous peptide antigen via MHC class I; response to yeast; protein targeting to membrane; inflammatory response; vascular endothelial growth factor receptor signaling pathway; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I
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Research Articles on NCF1

1. Direct contact of solid tumor cells and ECs activates endothelial NAD(P)H oxidase-mediated superoxide production. The oxidative stress contributes to EC apoptosis which in turn facilitates tumor cell extravasation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NCF1 antibody

 Reference Product  PubMed Publications
 CYBB antibody  >13 publications with NCF1 and CYBB
 NCF2 antibody  >11 publications with NCF1 and NCF2
 CYBA antibody  >8 publications with NCF1 and CYBA
 NCF4 antibody  >3 publications with NCF1 and NCF4

Pathways associated with anti-NCF1 antibody

 Products by Pathway  Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram

Diseases associated with anti-NCF1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >27 publications with NCF1 and Inflammation
 Autoimmune Diseases Antibodies  >20 publications with NCF1 and Autoimmune Diseases
 Arthritis, Rheumatoid Antibodies  >19 publications with NCF1 and Arthritis, Rheumatoid
 Arthritis, Experimental Antibodies  >18 publications with NCF1 and Arthritis, Experimental
 Nervous System Diseases Antibodies  >8 publications with NCF1 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >4 publications with NCF1 and Cardiovascular Diseases
 Congenital Abnormalities Antibodies  >4 publications with NCF1 and Congenital Abnormalities
 Asthma Antibodies  >3 publications with NCF1 and Asthma
 Lung Diseases Antibodies  >3 publications with NCF1 and Lung Diseases
 Vascular Diseases Antibodies  >2 publications with NCF1 and Vascular Diseases

Organs/Tissues associated with anti-NCF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with NCF1 and Blood
 Lung Antibodies  >3 publications with NCF1 and Lung
 Brain Antibodies  >3 publications with NCF1 and Brain
 Spleen Antibodies  >3 publications with NCF1 and Spleen
 Bone Antibodies  >2 publications with NCF1 and Bone
 Connective Tissue Antibodies  >2 publications with NCF1 and Connective Tissue
 Lymph Node Antibodies  >2 publications with NCF1 and Lymph Node
 Lymph Antibodies  >2 publications with NCF1 and Lymph
 Stomach Antibodies  >1 publications with NCF1 and Stomach
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