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Nitrotyrosine (NOS2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS611986

Nitric oxide (NO) is implicated in carcinogenesis(1), chronic infection, inflammation (2) and neurodegeneration (3). High levels of both superoxide and nitric oxide in these tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues… More details

$865 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Nitrotyrosine (NOS2), Polyclonal Antibody
Full Product Name
Nitrotyrosine (nTyr)
Product Gene Name
anti-NOS2 antibody
Product Synonym Names
Anti -Nitrotyrosine (nTyr)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. Does not crossreact with unmodified tyrosine or with phospho-tyrosine.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
131,208 Da
Chromosome Location
Chromosome: 19; AC_000176.1 (19758764..19790464, complement). Location: chromosome: 19
3D Structure
ModBase 3D Structure for Q27995
Immunogen
Synthetic nitro-tyrosine containing peptides coupled to KLH.

Product Categories/Family for anti-NOS2 antibody

Antibodies; Abs to Nitrotyrosine
Applications Tested/Suitable for anti-NOS2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-NOS2 antibody
Suitable for use in ELISA, Western Blot and Immunocytochemistry (ABC detection method).
Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunocytochemistry (ABC): 1:50
ELISA: 1:1000

Western Blot (WB) of anti-NOS2 antibody

Western Blot analysis of whole cell lysates of different cell lines untreated or treated with peroxynitrite, degraded peroxynitrite or pervanadate, using MBS611986.
Rabbit Anti-NOS2 Polyclonal Antibody – Cat# MBS611986

Testing Data of anti-NOS2 antibody

Rabbit Anti-NOS2 Polyclonal Antibody, Testing Data – Cat# MBS611986

Other Notes

Small volumes of anti-NOS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOS2 purchase

MBS611986 is an antibody targeting NOS2. Also known as Nitrotyrosine (nTyr). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NOS2. It may not necessarily be applicable to this product.

NCBI GI #
116003827
NCBI GeneID
282876
NCBI Accession #
NP_001070267.1
UniProt Primary Accession #
Q27995
UniProt Secondary Accession #
Q06Q84; Q27985
UniProt Related Accession #
Q27995
GenBank Nucleotide #
NM_001076799.1
NCBI Official Full Name
nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2, inducible<
NCBI Official Symbol
NOS2
NCBI Official Synonym Symbols
iNOS; NOS2A
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Synonym Protein Names
Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2
Protein Family
Nitric oxide synthase
UniProt Gene Name
NOS2
UniProt Synonym Gene Names
Inducible NOS; iNOS; NOSII
UniProt Entry Name
NOS2_BOVIN
NCBI Protein Information
nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)

UniProt Comments for NOS2

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.
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Research Articles on NOS2

1. Differential cell-specific and spatiotemporal expression of the EDN1 system and NOS in the bovine utero-placental unit may be associated with regulation of vascular and cellular functions during pregnancy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NOS2 antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram

Diseases associated with anti-NOS2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2258 publications with NOS2 and Inflammation
 Necrosis Antibodies  >1897 publications with NOS2 and Necrosis
 Sepsis Antibodies  >543 publications with NOS2 and Sepsis
 Intestinal Diseases Antibodies  >381 publications with NOS2 and Intestinal Diseases
 Liver Diseases Antibodies  >357 publications with NOS2 and Liver Diseases
 Reperfusion Injury Antibodies  >351 publications with NOS2 and Reperfusion Injury
 Hypertension Antibodies  >283 publications with NOS2 and Hypertension
 Endotoxemia Antibodies  >248 publications with NOS2 and Endotoxemia
 Shock, Septic Antibodies  >236 publications with NOS2 and Shock, Septic
 Kidney Diseases Antibodies  >214 publications with NOS2 and Kidney Diseases

Organs/Tissues associated with anti-NOS2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1149 publications with NOS2 and Muscle
 Intestine Antibodies  >459 publications with NOS2 and Intestine
 Ovary Antibodies  >47 publications with NOS2 and Ovary
 Extraembryonic Tissue Antibodies  >5 publications with NOS2 and Extraembryonic Tissue
 Uncharacterized Tissue Antibodies  >1 publications with NOS2 and Uncharacterized Tissue
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