Rabbit Anti-PLD1 Polyclonal Antibody – Cat# MBS612027

Western Blot analysis of extracts from HeLa cells, untreated ot PMA-treated for the indicated times, MBS612027 (upper) or P4074-50 (lower).

Phospholipase D1 (PLD1) Antibody – Rabbit Polyclonal

Name: PLD1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS612027
Price: 830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS612027

Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified.… More details

$830 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Phospholipase D1 (Pld1), Polyclonal Antibody

Full Product Name

Phospholipase D1, phosphorylated (Thr147) (PLD1)

Product Gene Name

anti-Pld1 antibody

Product Synonym Names

Anti -Phospholipase D1, phosphorylated (Thr147) (PLD1)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Purity/Purification

Affinity Purified
Purified by Protein A and peptide affinity chromatography.

Specificity

Detects endogenous levels of human PLD1 phosphorylated at Thr147. Does not crossreact with phosphorylated PLD2.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

123,814 Da

Chromosome Location

Chromosome: 2; NC_005101.2 (115306940..115460518). Location: 2q23.3-q24

3D Structure

ModBase 3D Structure for P70496

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr147 of human PLD1.

Product Categories/Family for anti-Pld1 antibody

Antibodies; Abs to Enzymes; Phospholipase

Applications Tested/Suitable for anti-Pld1 antibody

Western Blot (WB)

Application Notes for anti-Pld1 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Band is observed at ~120kD.

Other Notes

Small volumes of anti-Pld1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Pld1 purchase

MBS612027 is an antibody targeting Pld1. Also known as Phospholipase D1, phosphorylated (Thr147) (PLD1). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Pld1. It may not necessarily be applicable to this product.

NCBI GI #

25742819

NCBI GeneID

25096

NCBI Accession #

NP_112254.1

UniProt Primary Accession #

P70496

UniProt Secondary Accession #

O08959; O35856; O54765; P70497; Q9QWJ6

UniProt Related Accession #

P70496

GenBank Nucleotide #

NM_030992.1

NCBI Official Full Name

phospholipase D1

NCBI Official Synonym Full Names

phospholipase D1

NCBI Official Symbol

Pld1

NCBI Official Synonym Symbols

Plda; Pldb; Pld1a; Pld1b

UniProt Protein Name

Phospholipase D1

UniProt Synonym Protein Names

Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1

Protein Family

Pyridoxal 4-dehydrogenase

UniProt Gene Name

Pld1

UniProt Entry Name

PLD1_RAT

NCBI Protein Information

phospholipase D1; PLD 1; rPLD1; choline phosphatase 1; phosphatidylcholine-hydrolyzing phospholipase D1; Phoshpolipase D gene 1 probably with two splicing variants A and B; phospholipase D gene 1, probably with two splicing variants A and B

NCBI Summary for Pld1

ubiquitous enzyme that hydrolyzes phosphatidylcholine to phosphatidic acid (PA) and choline [RGD]

UniProt Comments for Pld1

PLD1: a phosphatidylcholine-hydrolyzing phospholipase. Activated by ADP-ribosylated Rho and protein kinase C. Implicated as a critical step in numerous cellular pathways including membrane trafficking and the regulation polymorphonuclear leukocyte degranulation and oxidant production. May be involved in the regulation of perinuclear intravesicular membrane traffic. Four splice-variant isoforms have been described.

Protein type: Phospholipase; EC 3.1.4.4; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; Golgi cisterna; intracellular membrane-bound organelle; lysosomal membrane; late endosome membrane; Golgi membrane; membrane; lamellipodium; perinuclear region of cytoplasm; endocytic vesicle; apical plasma membrane; vesicle; endosome

Molecular Function: protein binding; phospholipase D activity; catalytic activity; phosphoinositide binding

Biological Process: response to peptide hormone stimulus; defense response to Gram-positive bacterium; phospholipid catabolic process; metabolic process; phosphatidic acid biosynthetic process; regulation of microvillus biogenesis; phospholipid biosynthetic process; positive regulation of cell migration

Research Articles on Pld1

1. These results indicate that inhibition of PLD activity by CHS-111 is attributable to the blockade of RhoA activation via multiple steps in the signalling cascade.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Pld1 antibody

Products by Pathway	Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Ether Lipid Metabolism Pathway antibodies	Ether Lipid Metabolism Pathway Diagram
Ether Lipid Metabolism Pathway antibodies	Ether Lipid Metabolism Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Glutamatergic Synapse Pathway antibodies	Glutamatergic Synapse Pathway Diagram
Glutamatergic Synapse Pathway antibodies	Glutamatergic Synapse Pathway Diagram
Glycerophospholipid Metabolism Pathway antibodies	Glycerophospholipid Metabolism Pathway Diagram

Diseases associated with anti-Pld1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>50 publications with Pld1 and Neoplasms
Inflammation Antibodies	>15 publications with Pld1 and Inflammation
Carcinoma Antibodies	>13 publications with Pld1 and Carcinoma
Vascular Diseases Antibodies	>12 publications with Pld1 and Vascular Diseases
Cardiovascular Diseases Antibodies	>12 publications with Pld1 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>9 publications with Pld1 and Breast Neoplasms
Leukemia Antibodies	>9 publications with Pld1 and Leukemia
Neoplasms, Experimental Antibodies	>8 publications with Pld1 and Neoplasms, Experimental
Cell Transformation, Neoplastic Antibodies	>8 publications with Pld1 and Cell Transformation, Neoplastic
Necrosis Antibodies	>7 publications with Pld1 and Necrosis

Organs/Tissues associated with anti-Pld1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>53 publications with Pld1 and Brain
Lung Antibodies	>23 publications with Pld1 and Lung
Kidney Antibodies	>18 publications with Pld1 and Kidney
Ovary Antibodies	>16 publications with Pld1 and Ovary
Colon Antibodies	>8 publications with Pld1 and Colon
Eye Antibodies	>8 publications with Pld1 and Eye
Spleen Antibodies	>4 publications with Pld1 and Spleen
Small Intestine Antibodies	>3 publications with Pld1 and Small Intestine
Placenta Antibodies	>1 publications with Pld1 and Placenta
Dorsal Root Ganglion Antibodies	>1 publications with Pld1 and Dorsal Root Ganglion