Rabbit Anti-EAT-4 Polyclonal Antibody – Cat# MBS612098

EAT-4 Antibody – Rabbit Polyclonal

Name: EAT-4 Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS612098
Price: 905 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS612098

Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic… More details

$905 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EAT-4, Polyclonal Antibody

Full Product Name

Anti - EAT-4 (Vesicular Type Transporters for Glutamate, VGLUT)

Product Gene Name

anti-EAT-4 antibody

Product Synonym Names

EAT-4 (Vesicular Type Transporters for Glutamate, VGLUT)

Clonality

Polyclonal

Isotype

IgG

Host

Host: Rabbit
Source: Rat

Species Reactivity

Rat

Form/Format

Serum
Supplied as a liquid, neat serum, 40% glycerol.

Purity/Purification

Serum

Specificity

The peptide has no significant sequence with mammalian VGLUT1/2 or other NaPi-related (Type-1 to Type-III) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

63,109 Da

Chromosome Location

Chromosome: III; NC_003281.8 (9136827..9141733)

3D Structure

ModBase 3D Structure for P34644

Immunogen

An 18 AA synthetic peptide witin the N-terminus of C. elegans EAT-4 was synthesized, conjugated to KLH

Product Categories/Family for anti-EAT-4 antibody

Abs to Glucose Transporters

Applications Tested/Suitable for anti-EAT-4 antibody

ELISA, Western Blot

Application Notes for anti-EAT-4 antibody

Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. EAT-4 is ~65kD protein.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Product References and Citations for anti-EAT-4 antibody

• 1. Lee RYN et al (1999) J. Neurosci. 19:159-167

Other Notes

Small volumes of anti-EAT-4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EAT-4 purchase

MBS612098 is an antibody targeting EAT-4. Also known as Anti - EAT-4 (Vesicular Type Transporters for Glutamate, VGLUT). Reactive in Rat. Host: Rabbit
Source: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for EAT-4. It may not necessarily be applicable to this product.

NCBI GI #

71997452

NCBI GeneID

176291

NCBI Accession #

NP_499023.3

UniProt Primary Accession #

P34644

UniProt Secondary Accession #

Q9TZN7

UniProt Related Accession #

H88548

GenBank Nucleotide #

NM_066622.3

NCBI Official Full Name

EATing: abnormal pharyngeal pumping family member (eat-4)

NCBI Official Symbol

eat-4

UniProt Protein Name

Probable vesicular glutamate transporter eat-4

UniProt Synonym Protein Names

Abnormal pharyngeal pumping eat-4

Protein Family

Probable vesicular glutamate transporter

UniProt Gene Name

eat-4

UniProt Entry Name

EAT4_CAEEL

NCBI Protein Information

EATing: abnormal pharyngeal pumping family member (eat-4)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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