• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

KLF4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6121372

KLF4, which is also known as the gut-enriched Kruppel-like factor (GKLF), is a zinc finger-containing transcription factor that belongs to the Kruppel-like family of transcription factors. KLF4 plays important roles during the proliferation and… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLF4, Monoclonal Antibody
Full Product Name
KLF4 (Kruppel-like Factor 4, Epithelial Zinc Finger Protein, EZF, Gut-enriched Krueppel-like Factor, GKLF) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
56CT5.1.6
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human KLF4. Species crossreactivity: mouse
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
51kDa
Chromosome Location
Chromosome: 9; NC_000009.11 (110247133..110252047, complement). Location: 9q31
OMIM
602253
3D Structure
ModBase 3D Structure for O43474
Immunogen
Recombinant protein corresponding to human KLF4.
Conjugate
FITC
Special Handling
Light sensitive
Applications Tested/Suitable for anti-KLF4 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-KLF4 antibody
Applications are based on unconjugated antibody.

Testing Data of anti-KLF4 antibody

At 1:4000 dilution + Recombinant protein lysate lysates/proteins at 20ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5%
Mouse Anti-KLF4 Monoclonal Antibody – Cat# MBS6121372

Flow Cytometry (FC/FACS) of anti-KLF4 antibody

Flow cytometric analysis of MCF-7 cells using (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Mouse Anti-KLF4 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6121372

Immunofluorescence (IF) of anti-KLF4 antibody

Immunoflurescence analysis of (5ug/ml). Hela cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at ~62 hours after transfection.
Mouse Anti-KLF4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6121372

Product References and Citations for anti-KLF4 antibody

1. MCC-555-induced NAG-1 expression is mediated in part by KLF4. Cekanova M, et al. Eur J Pharmacol, 2010 Jul 10. PMID 20385121. Kruppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression. Yori JL, et al. J Biol Chem, 2010 May 28. PMID 20356845. MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. Tian Y, et al. J Biol Chem, 2010 Mar 12. PMID 20075075. Genetic and epigenetic inactivation of Kruppel-like factor 4 in medulloblastoma. Nakahara Y, et al. Neoplasia, 2010 Jan. PMID 20072650. Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression. Zhang P, et al. J Biol Chem, 2010 Mar 19. PMID 20071344.

Other Notes

Small volumes of anti-KLF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLF4 purchase

MBS6121372 is an antibody targeting KLF4. Also known as KLF4 (Kruppel-like Factor 4, Epithelial Zinc Finger Protein, EZF, Gut-enriched Krueppel-like Factor, GKLF) (FITC). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KLF4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Krueppel-like factor 4
NCBI Official Synonym Full Names
Kruppel-like factor 4 (gut)
NCBI Official Symbol
KLF4
NCBI Official Synonym Symbols
EZF; GKLF
UniProt Protein Name
Krueppel-like factor 4
UniProt Synonym Protein Names
Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
Protein Family
UniProt Gene Name
KLF4
UniProt Synonym Gene Names
EZF; GKLF
UniProt Entry Name
KLF4_HUMAN
NCBI Protein Information
Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000