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MAP Kinase 15 (MAPK15) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6121381

MBS6121381 is an antibody targeting MAPK15. Also known as MAP Kinase 15 (Mitogen-activated Protein Kinase 15, MAPK 15, MAPK15, Extracellular Signal-regulated Kinase 7, ERK7, ERK-7, Extracellular Signal-regulated Kinase 8, ERK8, ERK-8) (FITC). Reactive in… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAP Kinase 15 (MAPK15), Monoclonal Antibody
Full Product Name
MAP Kinase 15 (Mitogen-activated Protein Kinase 15, MAPK 15, MAPK15, Extracellular Signal-regulated Kinase 7, ERK7, ERK-7, Extracellular Signal-regulated Kinase 8, ERK8, ERK-8) (FITC)
Product Gene Name
anti-MAPK15 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
168CT10.6.6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein G Affinity Chromatography
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
59,832 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (144798507..144804633). Location: chromosome: 8
3D Structure
ModBase 3D Structure for Q8TD08
Immunogen
MAPK15 Mab is generated from mouse immunized with MAPK15 recombinant protein.
Conjugate
FITC
Special Handling
Light sensitive

Product Categories/Family for anti-MAPK15 antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-MAPK15 antibody
FLISA, Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-MAPK15 antibody
FLISA: 1:1000
IF: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MAPK15 antibody

MAPK15 Antibody western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MAPK15 antibody detected the MAPK15 protein (arrow).
Mouse Anti-MAPK15 Monoclonal Antibody – Cat# MBS6121381

Immunofluorescence (IF) of anti-MAPK15 antibody

Confocal immunofluorescent analysis of MAPK15 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Mouse Anti-MAPK15 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6121381

Other Notes

Small volumes of anti-MAPK15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAPK15. It may not necessarily be applicable to this product.

NCBI GI #
95147356
NCBI GeneID
225689
NCBI Accession #
NP_620590.2
UniProt Primary Accession #
Q8TD08
UniProt Secondary Accession #
Q2TCF9; Q8N362
UniProt Related Accession #
Q8TD08
GenBank Nucleotide #
NM_139021.2
NCBI Official Full Name
mitogen-activated protein kinase 15
NCBI Official Synonym Full Names
mitogen-activated protein kinase 15
NCBI Official Symbol
MAPK15
NCBI Official Synonym Symbols
ERK7; ERK8
UniProt Protein Name
Mitogen-activated protein kinase 15
UniProt Synonym Protein Names
Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8
Protein Family
Mitogen-activated protein kinase
UniProt Gene Name
MAPK15
UniProt Synonym Gene Names
ERK7; ERK8; MAP kinase 15; MAPK 15; ERK-7; ERK-8
UniProt Entry Name
MK15_HUMAN
NCBI Protein Information
mitogen-activated protein kinase 15; ERK-7; ERK-8; MAPK 15; MAP kinase 15; extracellular regulated kinase 8 delta; extracellular signal regulated kinase 8; extracellular signal-regulated kinase 7; extracellular signal-regulated kinase 8

UniProt Comments for MAPK15

ERK7: In vitro, phosphorylates MBP. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; Erk7 subfamily

Chromosomal Location of Human Ortholog: -

Cellular Component: extracellular region; intracellular; nucleus

Molecular Function: MAP kinase activity; SH3 domain binding; ATP binding

Biological Process: negative regulation of DNA replication; positive regulation of protein ubiquitination; protein amino acid autophosphorylation; positive regulation of protein catabolic process; MAPKKK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; response to estradiol stimulus
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Research Articles on MAPK15

1. ATG8-like proteins (MAP1LC3B, GABARAP and GABARAPL1) are novel interactors of MAPK15/ERK8, a MAP kinase involved in cell proliferation and transformation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAPK15 antibody

 Reference Product  PubMed Publications
 JUN antibody  >1 publications with MAPK15 and JUN

Organs/Tissues associated with anti-MAPK15 antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >1 publications with MAPK15 and Pancreas
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