• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

EZH2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6121671

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EZH2, Monoclonal Antibody
Full Product Name
EZH2 (EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6) (PE)
Product Gene Name
anti-EZH2 antibody
Product Synonym Names
EZH2; KMT6; Histone-lysine N-methyltransferase EZH2; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
144CT2.1.1.5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein G Affinity Chromatography
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze R-Phycoerythrin conjugates. Light sensitive.
Molecular Weight
746
Chromosome Location
Chromosome: 7; NC_000007.13 (148504464..148581441, complement). Location: 7q35-q36
OMIM
277590
3D Structure
ModBase 3D Structure for Q15910
Immunogen
EZH2 antibody is generated from mouse immunized with EZH2 recombinant protein.
Conjugate
PE
Special Handling
Light sensitive

Product Categories/Family for anti-EZH2 antibody

Antibodies; Enzymes; Methyltransferase
Applications Tested/Suitable for anti-EZH2 antibody
FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-EZH2 antibody
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
IF: 1:10-50
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-EZH2 antibody

Fluorescent image of U251 cell stained with EZH2 Antibody.U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EZH2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).EZH2 immunoreactivity is localized to Nucleus significantly.
Mouse Anti-EZH2 Monoclonal Antibody – Cat# MBS6121671

Western Blot (WB) of anti-EZH2 antibody

Western blot analysis of EZH2 antibody in T47D cell line lysates (35ug/lane).EZH2 (arrow) was detected using the purified Mab.
Mouse Anti-EZH2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6121671

Immunohistochemistry (IHC) of anti-EZH2 antibody

EZH2 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Mouse Anti-EZH2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6121671

Product References and Citations for anti-EZH2 antibody

Long noncoding RNA as modular scaffold of histone modification complexes. Tsai MC, et al. Science, 2010 Aug 6. PMID 20616235. Implications of enhancer of zeste homologue 2 expression in pancreatic ductal adenocarcinoma. Toll AD, et al. Hum Pathol, 2010 Sep. PMID 20573371. Distinctive expression of the polycomb group proteins Bmi1 polycomb ring finger oncogene and enhancer of zeste homolog 2 in nonsmall cell lung cancers and their clinical and clinicopathologic significance. Kikuchi J, et al. Cancer, 2010 Jun 15. PMID 20564407. EZH2 and STAT6 expression profiles are correlated with colorectal cancer stage and prognosis. Wang CG, et al. World J Gastroenterol, 2010 May 21. PMID 20480530. ETS transcription factors control transcription of EZH2 and epigenetic silencing of the tumor suppressor gene Nkx3.1 in prostate cancer. Kunderfranco P, et al. PLoS One, 2010 May 10. PMID 20479932.

Other Notes

Small volumes of anti-EZH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EZH2 purchase

MBS6121671 is an antibody targeting EZH2. Also known as EZH2 (EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6) (PE). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for EZH2. It may not necessarily be applicable to this product.

NCBI GI #
322506097
NCBI GeneID
2146
NCBI Accession #
NP_001190176.1
UniProt Primary Accession #
Q15910
UniProt Secondary Accession #
Q15755; Q75MG3; Q92857; Q96FI6; B2RAQ1; B3KS30; B7Z1D6; B7Z7L6
UniProt Related Accession #
Q15910
GenBank Nucleotide #
NM_001203247.1
NCBI Official Full Name
histone-lysine N-methyltransferase EZH2 isoform c
NCBI Official Synonym Full Names
enhancer of zeste homolog 2 (Drosophila)
NCBI Official Symbol
EZH2
NCBI Official Synonym Symbols
WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
UniProt Protein Name
Histone-lysine N-methyltransferase EZH2
UniProt Synonym Protein Names
ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
EZH2
UniProt Synonym Gene Names
KMT6
UniProt Entry Name
EZH2_HUMAN
NCBI Protein Information
histone-lysine N-methyltransferase EZH2; lysine N-methyltransferase 6

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EZH2 antibody

 Reference Product  PubMed Publications
 EED antibody  >118 publications with EZH2 and EED
 SUZ12 antibody  >102 publications with EZH2 and SUZ12
 ASXL1 antibody  >50 publications with EZH2 and ASXL1
 EZH1 antibody  >29 publications with EZH2 and EZH1
 HDAC1 antibody  >19 publications with EZH2 and HDAC1
 HDAC2 antibody  >9 publications with EZH2 and HDAC2
 CDK1 antibody  >8 publications with EZH2 and CDK1
 PHF1 antibody  >6 publications with EZH2 and PHF1
 RBBP4 antibody  >3 publications with EZH2 and RBBP4
 VAV1 antibody  >2 publications with EZH2 and VAV1

Pathways associated with anti-EZH2 antibody

 Products by Pathway  Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MiRs In Muscle Cell Differentiation Pathway antibodies  MiRs In Muscle Cell Differentiation Pathway Diagram

Diseases associated with anti-EZH2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >527 publications with EZH2 and Neoplasms
 Prostatic Neoplasms Antibodies  >89 publications with EZH2 and Prostatic Neoplasms
 Breast Neoplasms Antibodies  >87 publications with EZH2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >83 publications with EZH2 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >62 publications with EZH2 and Neoplasm Metastasis
 Liver Diseases Antibodies  >33 publications with EZH2 and Liver Diseases
 Liver Neoplasms Antibodies  >32 publications with EZH2 and Liver Neoplasms
 Myelodysplastic Syndromes Antibodies  >31 publications with EZH2 and Myelodysplastic Syndromes
 Carcinoma, Hepatocellular Antibodies  >30 publications with EZH2 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >28 publications with EZH2 and Neoplasms, Experimental

Organs/Tissues associated with anti-EZH2 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >50 publications with EZH2 and Embryonic Tissue
 Brain Antibodies  >42 publications with EZH2 and Brain
 Bone Marrow Antibodies  >34 publications with EZH2 and Bone Marrow
 Vascular Antibodies  >15 publications with EZH2 and Vascular
 Placenta Antibodies  >6 publications with EZH2 and Placenta
 Testis Antibodies  >5 publications with EZH2 and Testis
 Mixed Antibodies  >4 publications with EZH2 and Mixed
 Thymus Antibodies  >2 publications with EZH2 and Thymus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000