Mouse Anti-MyoD1 Monoclonal Antibody – Cat# MBS6123531

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human rhabdomyosarcoma using 168841-AF.

MyoD1 Antibody – Mouse Monoclonal

Name: MyoD1 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS6123531
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6123531

MBS6123531 is an antibody targeting MyoD1. Also known as MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (PE). Reactive in Chicken, Human,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MyoD1, Monoclonal Antibody

Full Product Name

MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (PE)

Product Gene Name

anti-MyoD1 antibody

Product Synonym Names

bHLHc1; Class C basic helix-loop-helix protein 1; Myoblast determination protein 1; Myogenic differentiation 1; Myogenic factor 3 (Myf-3); Myogenin D1; PUM

Clonality

Monoclonal

Isotype

IgG1,k

Clone Number

5.8A, MYD712

Host

Mouse

Species Reactivity

Chicken, Human, Mouse, Rat

Form/Format

Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).

Purity/Purification

Purified by Protein A/G Affinity Chromatography.

Specificity

Recognizes the phosphor-protein, human MyoD1 at 45kD. Labels the nuclei of myoblasts in developing muscle tissues. Species Crossreactivity: mouse, rat, chicken. Does not react with myogenin, Myf5, or Myf6.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

34,501 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (17741110..17743678). Location: 11p15.4

OMIM

159970

3D Structure

ModBase 3D Structure for P15172

Immunogen

Recombinant mouse MyoD1 protein (5.8A) and Recombinant human MyoD1 protein (MYD712). Cellular Localization: Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentatiation.

Conjugate

Positive Control

Rhabdomyosarcoma

Special Handling

Light sensitive

Product Categories/Family for anti-MyoD1 antibody

Antibodies; Transcription Factors

Applications Tested/Suitable for anti-MyoD1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Application Notes for anti-MyoD1 antibody

FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
Applications are based on unconjugated antibody.

Product References and Citations for anti-MyoD1 antibody

1. Tallini G; Parham DM; Dias P; Cordon-Cardo C; Houghton PJ; Rosai J. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994, 144(4):693-701. 2. Dias P; Parham DM; Shapiro DN; Tapscott SJ; Houghton PJ. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9. 3. Rosai J; Dias P; Parham DM; Shapiro DN; Houghton P. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. Am Journal of Surgical Pathology, 1991, 15(10):974-81.

Other Notes

Small volumes of anti-MyoD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MyoD1. It may not necessarily be applicable to this product.

NCBI GI #

23111009

NCBI GeneID

4654

NCBI Accession #

NP_002469.2

UniProt Primary Accession #

P15172

UniProt Secondary Accession #

P10085

UniProt Related Accession #

P15172

GenBank Nucleotide #

NM_002478.4

NCBI Official Full Name

myoblast determination protein 1

NCBI Official Synonym Full Names

myogenic differentiation 1

NCBI Official Symbol

MYOD1

NCBI Official Synonym Symbols

PUM; MYF3; MYOD; bHLHc1

UniProt Protein Name

Myoblast determination protein 1

UniProt Synonym Protein Names

Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3

Protein Family

Myoblast determination protein

UniProt Gene Name

MYOD1

UniProt Synonym Gene Names

BHLHC1; MYF3; MYOD; bHLHc1; Myf-3

UniProt Entry Name

MYOD1_HUMAN

NCBI Protein Information

myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1

NCBI Summary for MyoD1

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

UniProt Comments for MyoD1

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; RNA splicing; Transcription factor

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome

Research Articles on MyoD1

1. SREBP-1 regulate muscle protein synthesis through the downregulation of the expression of MYOD1, MYOG and MEF2C factors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MyoD1 antibody

Reference Product	PubMed Publications
JUN antibody	>66 publications with MyoD1 and JUN
TCF3 antibody	>12 publications with MyoD1 and TCF3
ID1 antibody	>8 publications with MyoD1 and ID1
SRF antibody	>7 publications with MyoD1 and SRF
RB1 antibody	>6 publications with MyoD1 and RB1
HDAC1 antibody	>5 publications with MyoD1 and HDAC1
SP1 antibody	>5 publications with MyoD1 and SP1
CDK4 antibody	>4 publications with MyoD1 and CDK4
SMAD3 antibody	>4 publications with MyoD1 and SMAD3
MEF2A antibody	>4 publications with MyoD1 and MEF2A

Pathways associated with anti-MyoD1 antibody

Products by Pathway	Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
CDO In Myogenesis Pathway antibodies	CDO In Myogenesis Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Id Signaling Pathway antibodies	Id Signaling Pathway Diagram
Myogenesis Pathway antibodies	Myogenesis Pathway Diagram
Notch-mediated HES/HEY Network Pathway antibodies	Notch-mediated HES/HEY Network Pathway Diagram
Regulation Of Nuclear SMAD2/3 Signaling Pathway antibodies	Regulation Of Nuclear SMAD2/3 Signaling Pathway Diagram
Regulation Of Retinoblastoma Protein Pathway antibodies	Regulation Of Retinoblastoma Protein Pathway Diagram
Signaling Events Mediated By HDAC Class III Pathway antibodies	Signaling Events Mediated By HDAC Class III Pathway Diagram
MiRs In Muscle Cell Differentiation Pathway antibodies	MiRs In Muscle Cell Differentiation Pathway Diagram

Diseases associated with anti-MyoD1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>202 publications with MyoD1 and Neoplasms
Nervous System Diseases Antibodies	>76 publications with MyoD1 and Nervous System Diseases
Disease Models, Animal Antibodies	>27 publications with MyoD1 and Disease Models, Animal
Necrosis Antibodies	>26 publications with MyoD1 and Necrosis
Muscular Atrophy Antibodies	>16 publications with MyoD1 and Muscular Atrophy
Inflammation Antibodies	>14 publications with MyoD1 and Inflammation
Breast Neoplasms Antibodies	>11 publications with MyoD1 and Breast Neoplasms
Lung Neoplasms Antibodies	>11 publications with MyoD1 and Lung Neoplasms
Adenocarcinoma Antibodies	>10 publications with MyoD1 and Adenocarcinoma
Heart Diseases Antibodies	>10 publications with MyoD1 and Heart Diseases

Organs/Tissues associated with anti-MyoD1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>603 publications with MyoD1 and Muscle
Heart Antibodies	>38 publications with MyoD1 and Heart
Eye Antibodies	>10 publications with MyoD1 and Eye
Lymph Antibodies	>8 publications with MyoD1 and Lymph
Placenta Antibodies	>2 publications with MyoD1 and Placenta