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Cytokeratin (KRT) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6124162

MBS6124162 is an antibody targeting KRT. Also known as Cytokeratin, pan (KRT) (AP). Reactive in Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat. Host: Mouse. More details

$980 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin (KRT), Monoclonal Antibody
Full Product Name
Cytokeratin, pan (KRT) (AP)
Product Gene Name
Product Synonym Names
KRT
Clonality
Monoclonal
Isotype
IgG1
Clone Number
SPM115 + SPM116
Host
Mouse
Species Reactivity
Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes Cytokeratin, pan. Species Crossreactivity: Human, Monkey, Bovine, Canine, Rabbit, Mouse, Rat, Chicken.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Molecular Weight
66039 MW
OMIM
M98776 Genomic DNA
3D Structure
ModBase 3D Structure for P04264
Immunogen
Human epidermal keratin. Cellular Localization: Cytoplasmic
Conjugate
AP
Positive Control
Skin, Adeno- or Squamous carcinomas

Product Categories/Family for anti-KRT antibody

Applications Tested/Suitable for anti-KRT antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-KRT antibody
IHC: 0.5-1ug/ml for 30 min at RT
WB: 0.5-1ug/ml for 2 hours at RT
IF: 1-2ug/ml
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-KRT antibody

Formalin-paraffin colon carcinoma stained with pan Cytokeratin Ab cocktail (SPM115+SPM116).
Mouse Anti-KRT Monoclonal Antibody – Cat# MBS6124162

Immunohistochemistry (IHC) of anti-KRT antibody

Formalin-paraffin colon carcinoma stained with pan Cytokeratin Ab cocktail (SPM115+SPM116).
Mouse Anti-KRT Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6124162

Product References and Citations for anti-KRT antibody

1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. 2. Tseng SCG et. al. Cell 1982; 30361.

Other Notes

Small volumes of anti-KRT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type II cytoskeletal 1
NCBI Official Synonym Full Names
keratin 1
NCBI Official Symbol
KRT1
NCBI Official Synonym Symbols
K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
UniProt Protein Name
Keratin, type II cytoskeletal 1
UniProt Synonym Protein Names
67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1
Protein Family
UniProt Gene Name
KRT1
UniProt Synonym Gene Names
KRTA; CK-1; K1
NCBI Protein Information
keratin, type II cytoskeletal 1

NCBI Summary for KRT

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT antibody

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