Rabbit Anti-BNIP3 Polyclonal Antibody – Cat# MBS613003

BNIP3 Antibody – Rabbit Polyclonal

Name: BNIP3 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS613003
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS613003

BNIP3, formerly NIP3 (nineteenkD interacting protein-3), is a pro-apoptotic, mitochondrial protein. It represents a subfamily of Bcl 2 related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and… More details

$625 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BNIP3, Polyclonal Antibody

Full Product Name

BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3)

Product Gene Name

anti-BNIP3 antibody

Product Synonym Names

Anti -BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3); anti-BCL2/adenovirus E1B 19kD protein-interacting protein 3 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in tris-citrate/phosphate [pH 7-8], 0.1% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human BNIP3. Species Crossreactivity: 93% homology with feline,

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

21,541 Da

Chromosome Location

Chromosome: 10; NC_000010.10 (133781204..133795435, complement). Location: 10q26.3

OMIM

603293

3D Structure

ModBase 3D Structure for Q12983

Immunogen

Synthetic peptide corresponding to a region between aa1 and 50 of human BNIP3 protein.

Cellular Localization

Mitochondrion. Co-expression with the EIB 19kD protein results in a shift in NIP3 localization to the nuclear envelope.

Positive Control

Hypoxic Hep3b lysates

Product Categories/Family for anti-BNIP3 antibody

Antibodies; Abs to Apoptosis

Applications Tested/Suitable for anti-BNIP3 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-BNIP3 antibody

Suitable for use in ELISA, Western Blotting.
Dilution: Western Blot: 1:500-1:1,000. Hypoxic samples at ~30 and 60kD, representing the BNIP3 protein. There are some non-specific bands present, as well. It is recommended that normoxic samples are run next to the hypoxic samples, as a negative control.

Other Notes

Small volumes of anti-BNIP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BNIP3 purchase

MBS613003 is an antibody targeting BNIP3. Also known as BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BNIP3. It may not necessarily be applicable to this product.

NCBI GI #

7669481

NCBI GeneID

664

NCBI Accession #

NP_004043.2

UniProt Primary Accession #

Q12983

UniProt Secondary Accession #

O14620; Q96GP0

UniProt Related Accession #

Q12983; Q53HF4; Q66K24; Q6NVY4

GenBank Nucleotide #

NM_004052.2

NCBI Official Full Name

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

NCBI Official Synonym Full Names

BCL2/adenovirus E1B 19kDa interacting protein 3

NCBI Official Symbol

BNIP3

NCBI Official Synonym Symbols

NIP3

UniProt Protein Name

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Protein Family

BCL2/adenovirus E1B 19 kDa protein-interacting protein

UniProt Gene Name

BNIP3

UniProt Synonym Gene Names

NIP3

UniProt Entry Name

BNIP3_HUMAN

NCBI Protein Information

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; OTTHUMP00000020752

NCBI Summary for BNIP3

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq]

UniProt Comments for BNIP3

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytoplasm; mitochondrial membrane; dendrite; nuclear envelope; nucleus; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; protein binding; GTPase binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: cell death; positive regulation of protein complex disassembly; induction of apoptosis by granzyme; viral reproduction; positive regulation of apoptosis; apoptosis; neuron apoptosis; positive regulation of programmed cell death; response to hyperoxia; autophagic cell death; response to hypoxia; brown fat cell differentiation; regulation of mitochondrial membrane permeability; mitochondrial fragmentation during apoptosis; negative regulation of membrane potential; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis

Research Articles on BNIP3

1. The high expression of HIF-1alpha and BNIP3 were closely related in laryngeal carcinomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BNIP3 antibody

Reference Product	PubMed Publications
BNIP3L antibody	>70 publications with BNIP3 and BNIP3L
BCL2 antibody	>43 publications with BNIP3 and BCL2
BCL2L1 antibody	>22 publications with BNIP3 and BCL2L1
CD47 antibody	>3 publications with BNIP3 and CD47

Pathways associated with anti-BNIP3 antibody

Products by Pathway	Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
MTOR Signaling Pathway antibodies	MTOR Signaling Pathway Diagram

Diseases associated with anti-BNIP3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>135 publications with BNIP3 and Neoplasms
Carcinoma Antibodies	>52 publications with BNIP3 and Carcinoma
Cardiovascular Diseases Antibodies	>44 publications with BNIP3 and Cardiovascular Diseases
Anoxia Antibodies	>40 publications with BNIP3 and Anoxia
Adenocarcinoma Antibodies	>34 publications with BNIP3 and Adenocarcinoma
Vascular Diseases Antibodies	>31 publications with BNIP3 and Vascular Diseases
Colorectal Neoplasms Antibodies	>22 publications with BNIP3 and Colorectal Neoplasms
Intestinal Diseases Antibodies	>20 publications with BNIP3 and Intestinal Diseases
Breast Neoplasms Antibodies	>19 publications with BNIP3 and Breast Neoplasms
Heart Failure Antibodies	>16 publications with BNIP3 and Heart Failure

Organs/Tissues associated with anti-BNIP3 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>58 publications with BNIP3 and Muscle
Heart Antibodies	>57 publications with BNIP3 and Heart
Blood Antibodies	>36 publications with BNIP3 and Blood
Brain Antibodies	>36 publications with BNIP3 and Brain
Vascular Antibodies	>35 publications with BNIP3 and Vascular
Liver Antibodies	>17 publications with BNIP3 and Liver
Lung Antibodies	>15 publications with BNIP3 and Lung
Bone Antibodies	>12 publications with BNIP3 and Bone
Kidney Antibodies	>8 publications with BNIP3 and Kidney
Prostate Antibodies	>8 publications with BNIP3 and Prostate