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ARHGEF6 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6130054

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARHGEF6, Monoclonal Antibody
Full Product Name
ARHGEF6 (COOL2, KIAA0006, PIXA, Rho Guanine Nucleotide Exchange Factor 6, Alpha-Pix, COOL-2, PAK-interacting Exchange Factor alpha, Rac/Cdc42 Guanine Nucleotide Exchange Factor 6) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2E5
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ARHGEF6.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa61-175 from ARHGEF6 (AAH39856) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
QTEADCINNINDFLKGCATLQVEIFDPDDLYSGVNFSKVLSTLLAVNKATEDQLSERPCGRSSSLSAANTSQTNPQGAVSSTVSGLQRQSKTVEMTENGSHQLIV
Conjugate
AP

Product Categories/Family for anti-ARHGEF6 antibody

Applications Tested/Suitable for anti-ARHGEF6 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-ARHGEF6 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ARHGEF6 antibody

Western Blot detection against Immunogen (38.06kD).
Mouse Anti-ARHGEF6 Monoclonal Antibody – Cat# MBS6130054

Western Blot (WB) of anti-ARHGEF6 antibody

Western Blot analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody. Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5kD). Lane 2: Non-transfected lysate.
Mouse Anti-ARHGEF6 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6130054

Immunoprecipitation (IP) of anti-ARHGEF6 antibody

Immunoprecipitation of ARHGEF6 transfected lysate using ARHGEF6 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ARHGEF6 monoclonal antibody.
Mouse Anti-ARHGEF6 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6130054

Testing Data of anti-ARHGEF6 antibody

Detection limit for recombinant GST tagged ARHGEF6 is ~0.03ng/ml as a capture antibody.
Mouse Anti-ARHGEF6 Monoclonal Antibody, Testing Data – Cat# MBS6130054

Testing Data of anti-ARHGEF6 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC42 and ARHGEF6 HeLa cells were stained with CDC42 rabbit purified polyclonal 1:1200 and ARHGEF6 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Mouse Anti-ARHGEF6 Monoclonal Antibody, Testing Data – Cat# MBS6130054

Other Notes

Small volumes of anti-ARHGEF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARHGEF6 purchase

MBS6130054 is an antibody targeting ARHGEF6. Also known as ARHGEF6 (COOL2, KIAA0006, PIXA, Rho Guanine Nucleotide Exchange Factor 6, Alpha-Pix, COOL-2, PAK-interacting Exchange Factor alpha, Rac/Cdc42 Guanine Nucleotide Exchange Factor 6) (AP). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGEF6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6, mRNA
NCBI Official Synonym Full Names
Rac/Cdc42 guanine nucleotide exchange factor 6
NCBI Official Symbol
ARHGEF6
NCBI Official Synonym Symbols
PIXA; COOL2; MRX46; Cool-2; alphaPIX; alpha-PIX
NCBI Protein Information
rho guanine nucleotide exchange factor 6

NCBI Summary for ARHGEF6

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific cognitive disability. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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