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B4GALNT1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6130165

GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
B4GALNT1, Monoclonal Antibody
Full Product Name
B4GALNT1 (Beta-1, 4-N-Acetyl-Galactosaminyl Transferase 1, GALGT, GALNACT) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
5F9
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human B4GALNT1. Species Crossreactivity: rat.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
59kDa
OMIM
601873
3D Structure
ModBase 3D Structure for Q00973
Immunogen
Partial recombinant corresponding to aa30-127 from human B4GALNT1 (NP_001469) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
APGLRLPLAPWAPPQSPRRPELPDLAPEPRYAHIPVRIKEQVVGLLAWNNCSCESSGGGLPLPFQKQVRAIDLTKAFDPAELRAASATREQEFQAFLS
Conjugate
AP

Product Categories/Family for anti-B4GALNT1 antibody

Applications Tested/Suitable for anti-B4GALNT1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-B4GALNT1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-B4GALNT1 antibody

Western Blot detection against Immunogen (36.52kD).
Mouse Anti-B4GALNT1 Monoclonal Antibody – Cat# MBS6130165

Western Blot (WB) of anti-B4GALNT1 antibody

B4GALNT1 monoclonal antibody, Western Blot analysis of B4GALNT1 expression in HeLa.
Mouse Anti-B4GALNT1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6130165

Western Blot (WB) of anti-B4GALNT1 antibody

B4GALNT1 monoclonal antibody. Western Blot analysis of B4GALNT1 expression in PC-12.
Mouse Anti-B4GALNT1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6130165

Testing Data of anti-B4GALNT1 antibody

Detection limit for recombinant GST tagged B4GALNT1 is ~0.1ng/ml as a capture antibody.
Mouse Anti-B4GALNT1 Monoclonal Antibody, Testing Data – Cat# MBS6130165

Other Notes

Small volumes of anti-B4GALNT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for B4GALNT1 purchase

MBS6130165 is an antibody targeting B4GALNT1. Also known as B4GALNT1 (Beta-1, 4-N-Acetyl-Galactosaminyl Transferase 1, GALGT, GALNACT) (AP). Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for B4GALNT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
beta-1,4 N-acetylgalactosaminyltransferase 1 isoform 1
NCBI Official Synonym Full Names
beta-1,4-N-acetyl-galactosaminyltransferase 1
NCBI Official Symbol
B4GALNT1
NCBI Official Synonym Symbols
GALGT; SPG26; GALNACT; GalNAc-T
UniProt Protein Name
Beta-1,4 N-acetylgalactosaminyltransferase 1
UniProt Synonym Protein Names
(N-acetylneuraminyl)-galactosylglucosylceramide; GM2/GD2 synthase; GalNAc-T
UniProt Gene Name
B4GALNT1
UniProt Synonym Gene Names
GALGT; SIAT2
UniProt Entry Name
B4GN1_HUMAN
NCBI Protein Information
beta-1,4 N-acetylgalactosaminyltransferase 1

NCBI Summary for B4GALNT1

GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
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UniProt Comments for B4GALNT1

B4GALNT1: Involved in the biosynthesis of gangliosides GM2, GD2 and GA2. Belongs to the glycosyltransferase 2 family.

Protein type: EC 2.4.1.92; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; Transferase

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: membrane; plasma membrane; integral to Golgi membrane

Molecular Function: (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity

Biological Process: sequestering of lipid; carbohydrate metabolic process; lipid glycosylation; protein amino acid glycosylation; glycosphingolipid metabolic process; spermatogenesis; ganglioside biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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