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BANF1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6130181

MBS6130181 is an antibody targeting BANF1. Also known as BANF1 (Barrier-to-autointegration Factor, Breakpoint Cluster Region Protein 1, BAF, BCRG1, D14S1460, MGC111161) (AP). Reactive in Human. Host: Mouse. More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BANF1, Monoclonal Antibody
Full Product Name
BANF1 (Barrier-to-autointegration Factor, Breakpoint Cluster Region Protein 1, BAF, BCRG1, D14S1460, MGC111161) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3F10-4G12
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human BANF1.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
10,059 Da
Immunogen
Full length recombinant corresponding to aa1-90 from BANF1 (AAH05942) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL
Conjugate
AP

Product Categories/Family for anti-BANF1 antibody

Applications Tested/Suitable for anti-BANF1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-BANF1 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-BANF1 antibody

Western Blot detection against Immunogen (35.9kD).
Mouse Anti-BANF1 Monoclonal Antibody – Cat# MBS6130181

Western Blot (WB) of anti-BANF1 antibody

BANF1 monoclonal antibody 2 Western Blot analysis of BANF1 expression in Jurkat.
Mouse Anti-BANF1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6130181

Immunohistochemistry (IHC) of anti-BANF1 antibody

Immunoperoxidase of monoclonal antibody to BANF1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].
Mouse Anti-BANF1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6130181

Testing Data of anti-BANF1 antibody

Detection limit for recombinant GST tagged BANF1 is ~1ng/ml as a capture antibody.
Mouse Anti-BANF1 Monoclonal Antibody, Testing Data – Cat# MBS6130181

Other Notes

Small volumes of anti-BANF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BANF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens barrier to autointegration factor 1, mRNA
NCBI Official Synonym Full Names
barrier to autointegration factor 1
NCBI Official Symbol
BANF1
NCBI Official Synonym Symbols
BAF; NGPS; BCRP1; D14S1460
NCBI Protein Information
barrier-to-autointegration factor

NCBI Summary for BANF1

The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Jan 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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