• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

BCL2L1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6130199

MBS6130199 is an antibody targeting BCL2L1. Also known as BCL2L1 (Bcl-2-like Protein 1, Bcl2-L-1, Apoptosis Regulator Bcl-X, BCL2L, BCLX) (AP). Reactive in Human. Host: Mouse. More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BCL2L1, Monoclonal Antibody
Full Product Name
BCL2L1 (Bcl-2-like Protein 1, Bcl2-L-1, Apoptosis Regulator Bcl-X, BCL2L, BCLX) (AP)
Product Gene Name
anti-BCL2L1 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3E2-2A5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human BCL2L1.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-233 from human BCL2L1 (AAH19307) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK
Conjugate
AP

Product Categories/Family for anti-BCL2L1 antibody

Antibodies; Apoptosis
Applications Tested/Suitable for anti-BCL2L1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-BCL2L1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-BCL2L1 antibody

Western Blot detection against Immunogen (51.37kD).
Mouse Anti-BCL2L1 Monoclonal Antibody – Cat# MBS6130199

Immunofluorescence (IF) of anti-BCL2L1 antibody

Immunofluorescence of monoclonal antibody to BCL2L1 on A-431 cell. [antibody concentration 10ug/ml].
Mouse Anti-BCL2L1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6130199

Testing Data of anti-BCL2L1 antibody

Detection limit for recombinant GST tagged BCL2L1 is ~0.03ng/ml as a capture antibody.
Mouse Anti-BCL2L1 Monoclonal Antibody, Testing Data – Cat# MBS6130199

Testing Data of anti-BCL2L1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between BID and BCL2L1. HeLa cells were stained with BID rabbit purified polyclonal 1:1200 and BCL2L1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Mouse Anti-BCL2L1 Monoclonal Antibody, Testing Data – Cat# MBS6130199

Western Blot (WB) of anti-BCL2L1 antibody

Western Blot analysis of BCL2L1 expression in transfected 293T cell line by BCL2L1 monoclonal antibody. Lane 1: BCL2L1 transfected lysate (26kD). Lane 2: Non-transfected lysate.
Mouse Anti-BCL2L1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6130199

Product References and Citations for anti-BCL2L1 antibody

1. A BACH2/BCL2L1 fusion gene resulting from at (6; 20)(q15; q11. 2) chromosomal translocation in the lymphoma cell line BLUE-1. Turkmen S, Riehn M, Klopocki E, Molkentin M, Reinhardt R, Burmeister T.Genes Chromosomes Cancer. 2011 Jun;50(6):389-96. doi: 10.1002/gcc.20863. Epub 2011 Mar 15.

Other Notes

Small volumes of anti-BCL2L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BCL2L1. It may not necessarily be applicable to this product.

NCBI GI #
17939633
NCBI GeneID
598
NCBI Accession #
BC019307
NCBI Related Accession #
AAH19307
UniProt Related Accession #
Q07817
NCBI Official Full Name
Homo sapiens BCL2-like 1, mRNA
NCBI Official Synonym Full Names
BCL2 like 1
NCBI Official Symbol
BCL2L1
NCBI Official Synonym Symbols
BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
Protein Family
Bcl-2-like protein
NCBI Protein Information
bcl-2-like protein 1

NCBI Summary for BCL2L1

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]
Show more

Research Articles on BCL2L1

1. Bim contains two distinct binding sites for the anti-apoptotic proteins Bcl-XL and Bcl-2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000