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CLTC Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6130653

Clathrin-coated pits, intracellular transport vesicles involved in endocytosis and other membrane trafficking processes, are multi-component units. Clathrin, the most abundant protein in these vesicles, is formed of a basic assembly unit called a triskelion… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CLTC, Monoclonal Antibody
Full Product Name
CLTC (Clathrin Heavy Chain 1, Clathrin Heavy Chain on Chromosome 17, CLH-17, CLH17, CLTCL2, KIAA0034) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2E5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human CLTC.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
D21260 mRNA
3D Structure
ModBase 3D Structure for Q00610
Immunogen
Partial recombinant corresponding to aa232-340 from human CLTC (NP_004850) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITN
Conjugate
AP

Product Categories/Family for anti-CLTC antibody

Applications Tested/Suitable for anti-CLTC antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-CLTC antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CLTC antibody

Western Blot detection against Immunogen (37.73kD).
Mouse Anti-CLTC Monoclonal Antibody – Cat# MBS6130653

Testing Data of anti-CLTC antibody

Detection limit for recombinant GST tagged CLTC is 0.3ng/ml as a capture antibody.
Mouse Anti-CLTC Monoclonal Antibody, Testing Data – Cat# MBS6130653

Testing Data of anti-CLTC antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between HIP1 and CLTC. HeLa cells were stained with HIP1 rabbit purified polyclonal 1:1200 and CLTC mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Mouse Anti-CLTC Monoclonal Antibody, Testing Data – Cat# MBS6130653

Other Notes

Small volumes of anti-CLTC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CLTC purchase

MBS6130653 is an antibody targeting CLTC. Also known as CLTC (Clathrin Heavy Chain 1, Clathrin Heavy Chain on Chromosome 17, CLH-17, CLH17, CLTCL2, KIAA0034) (AP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CLTC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
clathrin heavy chain 1 isoform 1
NCBI Official Synonym Full Names
clathrin heavy chain
NCBI Official Symbol
CLTC
NCBI Official Synonym Symbols
Hc; CHC; CHC17; MRD56; CLH-17; CLTCL2
UniProt Protein Name
Clathrin heavy chain 1
UniProt Synonym Protein Names
Clathrin heavy chain on chromosome 17; CLH-17
Protein Family
UniProt Gene Name
CLTC
UniProt Synonym Gene Names
CLH17; CLTCL2; KIAA0034; CLH-17
NCBI Protein Information
clathrin heavy chain 1

NCBI Summary for CLTC

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
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UniProt Comments for CLTC

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CLTC antibody

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