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C-Erbb2, P185 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS613098

c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor… More details

$560 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
c-erbB2, p185, Polyclonal Antibody
Full Product Name
c-erbB2, p185 (HER2, neu Receptor)
Product Synonym Names
Anti -c-erbB2, p185 (HER2, neu Receptor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Detects endogenous levels of total HER2 protein.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthesized non-phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 1221/1222 (N-L-YP-YP -W)
Applications Tested/Suitable for anti-c-erbB2, p185 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-c-erbB2, p185 antibody
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Immunohistochemistry (IHC) of anti-c-erbB2, p185 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MBS613098
Rabbit Anti-C-Erbb2, P185 Polyclonal Antibody – Cat# MBS613098

Immunofluorescence (IF) of anti-c-erbB2, p185 antibody

Immunofluorescence staining of methanol-fixed MCF cells using MBS613098
Rabbit Anti-C-Erbb2, P185 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS613098

Western Blot (WB) of anti-c-erbB2, p185 antibody

Western blot analysis of extract from SK-OV3 cells using HER2 (Ab-1221,1222) (lane 1 and 2) and HER2 (Phospho-Tyr1221, 1222) (lane 3 and 4)
Rabbit Anti-C-Erbb2, P185 Polyclonal Antibody, Western Blot (WB) – Cat# MBS613098

Other Notes

Small volumes of anti-c-erbB2, p185 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for c-erbB2, p185 purchase

MBS613098 is an antibody targeting c-erbB2, p185. Also known as c-erbB2, p185 (HER2, neu Receptor). Reactive in Human. Host: Rabbit.
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