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E2IG3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6131042

MBS6131042 is an antibody targeting E2IG3. Also known as E2IG3 (Nucleostemin, GNL3, Guanine Nucleotide-binding Protein-Like 3, Nucleolar GTP-binding Protein 3, Nucleostemin, E2-induced Gene 3 Protein, Novel Nucleolar Protein 47, NNP47, NS) (AP). Reactive in… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
E2IG3, Monoclonal Antibody
Full Product Name
E2IG3 (Nucleostemin, GNL3, Guanine Nucleotide-binding Protein-Like 3, Nucleolar GTP-binding Protein 3, Nucleostemin, E2-induced Gene 3 Protein, Novel Nucleolar Protein 47, NNP47, NS) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1A1
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human GNL3.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
60,540 Da
Immunogen
Full length recombinant corresponding to aa328-427 from human GNL3 (AAH01024) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
IEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGF
Conjugate
AP

Product Categories/Family for anti-E2IG3 antibody

Applications Tested/Suitable for anti-E2IG3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-E2IG3 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-E2IG3 antibody

Western Blot detection against Immunogen (37kD).
Mouse Anti-E2IG3 Monoclonal Antibody – Cat# MBS6131042

Western Blot (WB) of anti-E2IG3 antibody

Western Blot analysis of GNL3 expression in transfected 293T cell line by GNL3 monoclonal antibody. Lane 1: GNL3 transfected lysate (Predicted MW: 62kD). Lane 2: Non-transfected lysate.
Mouse Anti-E2IG3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6131042

Testing Data of anti-E2IG3 antibody

Detection limit for recombinant GST tagged GNL3 is 0.1ng/ml as a capture antibody.
Mouse Anti-E2IG3 Monoclonal Antibody, Testing Data – Cat# MBS6131042

Other Notes

Small volumes of anti-E2IG3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for E2IG3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens guanine nucleotide binding protein-like 3 (nucleolar), mRNA
NCBI Official Synonym Full Names
G protein nucleolar 3
NCBI Official Symbol
GNL3
NCBI Official Synonym Symbols
NS; E2IG3; NNP47; C77032
NCBI Protein Information
guanine nucleotide-binding protein-like 3

NCBI Summary for E2IG3

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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