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GAK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6131404

GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GAK, Monoclonal Antibody
Full Product Name
GAK (Cyclin-G-associated Kinase, FLJ16629, FLJ40395, MGC99654) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4C10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human GAK.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
134,464 Da
Immunogen
Partial recombinant corresponding to aa151-250 from human GAK (AAH08668) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQP
Conjugate
AP

Product Categories/Family for anti-GAK antibody

Applications Tested/Suitable for anti-GAK antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-GAK antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GAK antibody

Western Blot detection against Immunogen (36.63kD).
Mouse Anti-GAK Monoclonal Antibody – Cat# MBS6131404

Western Blot (WB) of anti-GAK antibody

GAK monoclonal antibody, Western Blot analysis of GAK expression in HeLa NE.
Mouse Anti-GAK Monoclonal Antibody, Western Blot (WB) – Cat# MBS6131404

Testing Data of anti-GAK antibody

Detection limit for recombinant GST tagged GAK is ~0.03ng/ml as a capture antibody.
Mouse Anti-GAK Monoclonal Antibody, Testing Data – Cat# MBS6131404

Western Blot (WB) of anti-GAK antibody

GAD2 rabbit polyclonal antibody. Western Blot analysis of GAD2 expression in human pancreas.Western Blot analysis of GAK expression in transfected 293T cell line by GAK monoclonal antibody. Lane 1: GAK transfected lysate (Predicted MW: 44kD). Lane 2: Non-transfected lysate.
Mouse Anti-GAK Monoclonal Antibody, Western Blot (WB) – Cat# MBS6131404

Other Notes

Small volumes of anti-GAK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAK purchase

MBS6131404 is an antibody targeting GAK. Also known as GAK (Cyclin-G-associated Kinase, FLJ16629, FLJ40395, MGC99654) (AP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GAK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens cyclin G associated kinase, mRNA
NCBI Official Synonym Full Names
cyclin G associated kinase
NCBI Official Symbol
GAK
NCBI Official Synonym Symbols
DNAJ26; DNAJC26
NCBI Protein Information
cyclin-G-associated kinase

NCBI Summary for GAK

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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