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Protein Kinase C Beta1 (PRKCB) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS613159

PKC 1 is a 671 aa, 77kD member of the PKC subfamily of Ser/Thr family of protein kinases. PKC 1 is a Ca2+ -activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins. Human PKC 2 is an alternatively spliced isoform with… More details

$1090 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Protein Kinase C beta1 (Prkcb), Polyclonal Antibody
Full Product Name
Protein Kinase C beta1 (PKCb1)
Product Gene Name
anti-Prkcb antibody
Product Synonym Names
Anti -Protein Kinase C beta1 (PKCb1)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Human, Rat
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Purity/Purification
Affinity Purified
Purified by human PKCb1 affinity chromatography.
Specificity
Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
76,751 Da
Chromosome Location
Chromosome: 1; NC_005100.3 (199295067..199639140). Location: 1q36
3D Structure
ModBase 3D Structure for P68403
Immunogen
E. coli-derived rhPKCb1 (aa607-671)

Product Categories/Family for anti-Prkcb antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-Prkcb antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-Prkcb antibody
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions., Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1% cross-reactivity with rhPKC., Optimal dilutions should be determined by each laboratory for each application.

Other Notes

Small volumes of anti-Prkcb antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Prkcb purchase

MBS613159 is an antibody targeting Prkcb. Also known as Protein Kinase C beta1 (PKCb1). Reactive in Human, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for Prkcb. It may not necessarily be applicable to this product.

NCBI GI #
288806594
NCBI GeneID
25023
NCBI Accession #
NP_001165776.1
UniProt Primary Accession #
P68403
UniProt Secondary Accession #
P04410; P04411
UniProt Related Accession #
P68403
GenBank Nucleotide #
NM_001172305.1
NCBI Official Full Name
protein kinase C beta type isoform 2
NCBI Official Synonym Full Names
protein kinase C, beta
NCBI Official Symbol
Prkcb
NCBI Official Synonym Symbols
Pkcb; Prkcb1
UniProt Protein Name
Protein kinase C beta type
Protein Family
Protein kinase
UniProt Gene Name
Prkcb
UniProt Synonym Gene Names
Pkcb; Prkcb1; PKC-B; PKC-beta
UniProt Entry Name
KPCB_RAT
NCBI Protein Information
protein kinase C beta type; PKC-beta; protein kinase C beta I; protein kinase C beta II; protein kinase C, beta 1

NCBI Summary for Prkcb

plays a role in hexosamine biosynthesis pathway induced transcriptional regulation [RGD, Feb 2006]

UniProt Comments for Prkcb

PKCB: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic receptors. Activates the NF-kappaB signaling pathway in B cells after antigen-receptor signaling. Two splice-variant isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.13; Motility/polarity/chemotaxis; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Alpha subfamily

Cellular Component: centrosome; membrane; brush border membrane; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein kinase C binding; zinc ion binding; histone binding; calcium channel regulator activity; chromatin binding; ATP binding

Biological Process: response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; B cell activation; transcription, DNA-dependent; dibenzo-p-dioxin metabolic process; apoptosis; positive regulation of B cell receptor signaling pathway; negative regulation of insulin receptor signaling pathway; positive regulation of odontogenesis of dentine-containing teeth; regulation of dopamine secretion; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; B cell receptor signaling pathway; response to vitamin D; response to ethanol; regulation of growth; calcium ion transport; response to glucose stimulus; response to hypoxia
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Research Articles on Prkcb

1. iNOS expression is highly modulated in alveolar epithelial cells by LPS via a Ca(2)/PKCalpha-beta1 pathway independent of ATP signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Prkcb antibody

 Products by Pathway  Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
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Diseases associated with anti-Prkcb antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with Prkcb and Neoplasms
 Disease Models, Animal Antibodies  >2 publications with Prkcb and Disease Models, Animal
 Kidney Diseases Antibodies  >2 publications with Prkcb and Kidney Diseases
 Inflammation Antibodies  >2 publications with Prkcb and Inflammation
 Renal Insufficiency Antibodies  >1 publications with Prkcb and Renal Insufficiency
 Cardiovascular Diseases Antibodies  >1 publications with Prkcb and Cardiovascular Diseases
 Hyperglycemia Antibodies  >1 publications with Prkcb and Hyperglycemia

Organs/Tissues associated with anti-Prkcb antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >2 publications with Prkcb and Kidney
 Heart Antibodies  >2 publications with Prkcb and Heart
 Lung Antibodies  >1 publications with Prkcb and Lung
 Eye Antibodies  >1 publications with Prkcb and Eye
 Brain Antibodies  >1 publications with Prkcb and Brain
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