P2X2 Receptor (P2RX2) Antibody – Rabbit Polyclonal

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot (Reducing): 1:1000
Cell membrane extracts were examined by SDS-Page, transferred to a nylon membrane. Membranes were blocked for 1 hour at 4 C with 0.1% Tween 20 and 2.5% milk powder in PBS. Membranes were incubated with P1003-05D (1:500 in the same buffer overnight at 4 C. Membranes were rinsed and incubated withI1904-47H: IgG, H&L (HRP) Pab GtxRt for 1 hour at RT. Following rinsing, the membranes were processed using ECL
Immunohistochemistry: 1:1000. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. P1003-05D was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4 degree C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
Immunocytochemistry: 1:1000. P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.