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RAD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6133290

MBS6133290 is an antibody targeting RAD1. Also known as RAD1 (Cell Cycle Checkpoint Protein RAD1, hRAD1, DNA Repair Exonuclease Rad1 Homolog, Rad1-like DNA Damage Checkpoint Protein, REC1) (AP). Reactive in Human. Host: Mouse. More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAD1, Monoclonal Antibody
Full Product Name
RAD1 (Cell Cycle Checkpoint Protein RAD1, hRAD1, DNA Repair Exonuclease Rad1 Homolog, Rad1-like DNA Damage Checkpoint Protein, REC1) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
1G2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human RAD1.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
7,576 Da
Immunogen
Partial recombinant corresponding to aa1-90, from human RAD1 (AAH06837) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLL
Conjugate
AP
Applications Tested/Suitable for anti-RAD1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-RAD1 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RAD1 antibody

Western Blot analysis of RAD1 expression in transfected 293T cell line by RAD1 monoclonal antibody. Lane 1: RAD1 transfected lysate (32.43kD). Lane 2: Non-transfected lysate.
Mouse Anti-RAD1 Monoclonal Antibody – Cat# MBS6133290

Immunohistochemistry (IHC) of anti-RAD1 antibody

Immunoperoxidase of monoclonal antibody to RAD1 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3ug/ml]
Mouse Anti-RAD1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6133290

Testing Data of anti-RAD1 antibody

Detection limit for recombinant GST tagged RAD1 is ~0.3ng/ml as a capture antibody.
Mouse Anti-RAD1 Monoclonal Antibody, Testing Data – Cat# MBS6133290

Western Blot (WB) of anti-RAD1 antibody

Western blot analysis of RAD1 over-expressed 293 cell line, cotransfected with RAD1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-RAD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6133290

Other Notes

Small volumes of anti-RAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens RAD1 homolog (S. pombe), mRNA
NCBI Official Synonym Full Names
RAD1 checkpoint DNA exonuclease
NCBI Official Symbol
RAD1
NCBI Official Synonym Symbols
REC1; HRAD1
NCBI Protein Information
cell cycle checkpoint protein RAD1

NCBI Summary for RAD1

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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