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TIMP1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6134207

MBS6134207 is an antibody targeting TIMP1. Also known as TIMP1 (Tissue Inhibitor of Metalloproteinases 1, TIMP-1, TIMP, Metalloproteinase Inhibitor 1, Collagenase inhibitor, CLGI, Erythroid-potentiating Activity, EPA, Fibroblast Collagenase Inhibitor) (AP).… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TIMP1, Monoclonal Antibody
Full Product Name
TIMP1 (Tissue Inhibitor of Metalloproteinases 1, TIMP-1, TIMP, Metalloproteinase Inhibitor 1, Collagenase inhibitor, CLGI, Erythroid-potentiating Activity, EPA, Fibroblast Collagenase Inhibitor) (AP)
Product Gene Name
Product Synonym Names
EPO; HCI
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4D12
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TIMP1.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
23,171 Da
Immunogen
Full length recombinant corresponding to aa1-170 from human TIMP1 (AAH07097) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAPFEPLASGILLLLWLIAPSRACTCVPPHPQTAFCNSDLVIRAKFVGTPEVNQTTLYQRYEIKMTKMYKGFQALGDAADIRFVYTPAMESVCGYFHRSHNRSEEFLIAGKLQDGLLHITTCSFVAPWNSLSLAQRRGFTKTYTVGCEECTVFPCLSIPCKLQSGTHCL
Conjugate
AP
Applications Tested/Suitable for anti-TIMP1 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-TIMP1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TIMP1 antibody

Western Blot analysis of TIMP1 expression in transfected 293T cell line by TIMP1 monoclonal antibody. Lane 1: TIMP1 transfected lysate (23.2kD). Lane 2: Non-transfected lysate.
Mouse Anti-TIMP1 Monoclonal Antibody – Cat# MBS6134207

Immunoprecipitation (IP) of anti-TIMP1 antibody

Immunoprecipitation of TIMP1 transfected lysate using TIMP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TIMP1 rabbit polyclonal antibody.
Mouse Anti-TIMP1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6134207

Testing Data of anti-TIMP1 antibody

Detection limit for recombinant GST tagged TIMP1 is ~3ng/ml as a capture antibody.
Mouse Anti-TIMP1 Monoclonal Antibody, Testing Data – Cat# MBS6134207

Other Notes

Small volumes of anti-TIMP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TIMP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens TIMP metallopeptidase inhibitor 1, mRNA
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1
NCBI Official Symbol
TIMP1
NCBI Official Synonym Symbols
EPA; EPO; HCI; CLGI; TIMP; TIMP-1
NCBI Protein Information
metalloproteinase inhibitor 1

NCBI Summary for TIMP1

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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