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TIP47 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6134210

MBS6134210 is an antibody targeting TIP47. Also known as TIP47 (M6PRBP1, Perilipin-3, 47kD Mannose 6-phosphate Receptor-binding Protein, 47kD MPR-binding Protein, Cargo Selection Protein TIP47, Mannose-6-phosphate Receptor-binding Protein 1, Placental Protein… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TIP47, Monoclonal Antibody
Full Product Name
TIP47 (M6PRBP1, Perilipin-3, 47kD Mannose 6-phosphate Receptor-binding Protein, 47kD MPR-binding Protein, Cargo Selection Protein TIP47, Mannose-6-phosphate Receptor-binding Protein 1, Placental Protein 17, PP17, PLIN3) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2F7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human M6PRBP1.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
47kDa
Immunogen
Full-length recombinant protein corresponding to aa1-434 from M6PRBP1 (AAH01590) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHVKTVCDAAEKGVRTLTAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQALSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGPFAPGITEKAPEEKK
Conjugate
AP
Applications Tested/Suitable for anti-TIP47 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-TIP47 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TIP47 antibody

Western Blot detection against Immunogen (73.85kD).
Mouse Anti-TIP47 Monoclonal Antibody – Cat# MBS6134210

Western Blot (WB) of anti-TIP47 antibody

Western Blot analysis of PLIN3 expression in transfected 293T cell line by 134464 Lane 1: PLIN3 transfected lysate (Predicted MW: 47kD). Lane 2: Non-transfected lysate.
Mouse Anti-TIP47 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6134210

Immunohistochemistry (IHC) of anti-TIP47 antibody

Immunoperoxidase of 134464 (3ug/ml) on formalin-fixed paraffin-embedded human tonsil.
Mouse Anti-TIP47 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6134210

Immunoprecipitation (IP) of anti-TIP47 antibody

Immunoprecipitation of PLIN3 transfected lysate using 134464 and Protein A Magnetic Bead, and immunoblotted with 134464.
Mouse Anti-TIP47 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6134210

Testing Data of anti-TIP47 antibody

Detection limit for recombinant GST tagged PLIN3 is 3ng/ml as a capture antibody.
Mouse Anti-TIP47 Monoclonal Antibody, Testing Data – Cat# MBS6134210

Other Notes

Small volumes of anti-TIP47 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TIP47. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens mannose-6-phosphate receptor binding protein 1, mRNA
NCBI Official Synonym Full Names
perilipin 3
NCBI Official Symbol
PLIN3
NCBI Official Synonym Symbols
PP17; TIP47; M6PRBP1
Protein Family
NCBI Protein Information
perilipin-3

NCBI Summary for TIP47

Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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