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ALAS2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6135251

ALAS2 specifies an erythroid-specific mitochondrially located enzyme. It catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ALAS2, Monoclonal Antibody
Full Product Name
ALAS2 (5-aminolevulinate Synthase, Erythroid-specific, Mitochondrial, ALAS-E, 5-aminolevulinic acid Synthase 2, Delta-ALA Synthase 2, Delta-aminolevulinate Synthase 2, ALASE, ASB) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6C1
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ALAS2. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
63,487 Da
OMIM
300751
3D Structure
ModBase 3D Structure for Q5JZF5
Immunogen
Partial recombinant corresponding to aa1-100 from human ALAS2 (NP_000023) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFK
Conjugate
APC

Product Categories/Family for anti-ALAS2 antibody

Applications Tested/Suitable for anti-ALAS2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-ALAS2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ALAS2 antibody

Western Blot detection against Immunogen (36.74kD).
Mouse Anti-ALAS2 Monoclonal Antibody – Cat# MBS6135251

Western Blot (WB) of anti-ALAS2 antibody

ALAS2 monoclonal antibody Western Blot analysis of ALAS2 expression in Raw 264.7.
Mouse Anti-ALAS2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6135251

Western Blot (WB) of anti-ALAS2 antibody

Western Blot analysis of ALAS2 expression in transfected 293T cell line by ALAS2 monoclonal antibody. Lane 1: ALAS2 transfected lysate (64kD). Lane 2: Non-transfected lysate.
Mouse Anti-ALAS2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6135251

Testing Data of anti-ALAS2 antibody

Detection limit for recombinant GST tagged ALAS2 is ~0.1ng/ml as a capture antibody.
Mouse Anti-ALAS2 Monoclonal Antibody, Testing Data – Cat# MBS6135251

Product References and Citations for anti-ALAS2 antibody

1. Hypoxic Induction of Human Erythroid-Specific Delta-Aminolevulinate Synthase Mediated by Hypoxia-Inducible Factor 1. Zhang FL, Shen GM, Liu XL, Wang F, Zhao HL, Yu J, Zhang JW.Biochemistry. 2011 Jan 20.

Other Notes

Small volumes of anti-ALAS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALAS2 purchase

MBS6135251 is an antibody targeting ALAS2. Also known as ALAS2 (5-aminolevulinate Synthase, Erythroid-specific, Mitochondrial, ALAS-E, 5-aminolevulinic acid Synthase 2, Delta-ALA Synthase 2, Delta-aminolevulinate Synthase 2, ALASE, ASB) APC. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ALAS2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform a
NCBI Official Synonym Full Names
5'-aminolevulinate synthase 2
NCBI Official Symbol
ALAS2
NCBI Official Synonym Symbols
ASB; ANH1; XLSA; ALASE; XLDPP; XLEPP; ALAS-E
UniProt Protein Name
5-aminolevulinate synthase, erythroid-specific, mitochondrial
UniProt Synonym Protein Names
5-aminolevulinic acid synthase 2; Delta-ALA synthase 2; Delta-aminolevulinate synthase 2
UniProt Gene Name
ALAS2
UniProt Synonym Gene Names
ALASE; ASB; ALAS-E
UniProt Entry Name
HEM0_HUMAN
NCBI Protein Information
5-aminolevulinate synthase, erythroid-specific, mitochondrial; 5-aminolevulinic acid synthase 2; aminolevulinate, delta-, synthase 2; delta-ALA synthase 2; delta-ALA synthetase; delta-aminolevulinate synthase 2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ALAS2 antibody

 Reference Product  PubMed Publications
 ALAD antibody  >1 publications with ALAS2 and ALAD
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