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DCTD Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6136182

MBS6136182 is an antibody targeting DCTD. Also known as DCTD (Deoxycytidylate Deaminase, dCMP Deaminase) APC. Reactive in Human. Host: Mouse. More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DCTD, Monoclonal Antibody
Full Product Name
DCTD (Deoxycytidylate Deaminase, dCMP Deaminase) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
4B9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human DCTD.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
607638
3D Structure
ModBase 3D Structure for P32321
Immunogen
Partial recombinant corresponding to aa69-178 from human DCTD (NM_001921.2) with GST tag. MW of the GST tag alone is 26kD.
Conjugate
APC

Product Categories/Family for anti-DCTD antibody

Applications Tested/Suitable for anti-DCTD antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-DCTD antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DCTD antibody

Western Blot detection against Immunogen (37.84kD)
Mouse Anti-DCTD Monoclonal Antibody – Cat# MBS6136182

Western Blot (WB) of anti-DCTD antibody

Western Blot analysis of DCTD expression in transfected 293T cell line by DCTD monoclonal antibody. Lane 1: DCTD transfected lysate (20.016kD). Lane 2: Non-transfected lysate.
Mouse Anti-DCTD Monoclonal Antibody, Western Blot (WB) – Cat# MBS6136182

Immunohistochemistry (IHC) of anti-DCTD antibody

Immunoperoxidase of monoclonal antibody to DCTD on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml]
Mouse Anti-DCTD Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6136182

Western Blot (WB) of anti-DCTD antibody

Western blot analysis of DCTD over-expressed 293 cell line, cotransfected with DCTD Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DCTD monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-DCTD Monoclonal Antibody, Western Blot (WB) – Cat# MBS6136182

Other Notes

Small volumes of anti-DCTD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCTD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
deoxycytidylate deaminase isoform b
NCBI Official Synonym Full Names
dCMP deaminase
NCBI Official Symbol
DCTD
UniProt Protein Name
Deoxycytidylate deaminase
UniProt Synonym Protein Names
dCMP deaminase
UniProt Gene Name
DCTD
UniProt Entry Name
DCTD_HUMAN
NCBI Protein Information
deoxycytidylate deaminase

NCBI Summary for DCTD

The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for DCTD

DCTD: catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.

Protein type: Nucleotide Metabolism - pyrimidine; EC 3.5.4.12; Hydrolase

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: cytosol

Molecular Function: zinc ion binding; dCMP deaminase activity

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleotide metabolic process; nucleotide biosynthetic process; pyrimidine nucleoside biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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