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HK2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6136986

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HK2, Monoclonal Antibody
Full Product Name
HK2 (Hexokinase-2, Hexokinase Type II, HK II, HKII, HK2, HXK2, DKFZp686M1669, Muscle Form Hexokinase) APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4H1
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human HK2. Species Crossreactivity: rat.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
102,380 Da
Immunogen
Partial recombinant corresponding to aa818-917 from human HK2 (AAH21116) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
IVKEVCTVVARRAAQLCGAGMAAVVDRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLQSEDGSGKGAALITAVACRIREAGQR
Conjugate
APC

Product Categories/Family for anti-HK2 antibody

Applications Tested/Suitable for anti-HK2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-HK2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HK2 antibody

Western Blot detection against Immunogen (36.63kD).
Mouse Anti-HK2 Monoclonal Antibody – Cat# MBS6136986

Western Blot (WB) of anti-HK2 antibody

HK2 monoclonal antibody Western Blot analysis of HK2 expression in PC-12
Mouse Anti-HK2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6136986

Western Blot (WB) of anti-HK2 antibody

HK2 monoclonal antibody. Western Blot analysis of HK2 expression in A-431
Mouse Anti-HK2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6136986

Testing Data of anti-HK2 antibody

Detection limit for recombinant GST tagged HK2 is ~0.3ng/ml as a capture antibody.
Mouse Anti-HK2 Monoclonal Antibody, Testing Data – Cat# MBS6136986

Product References and Citations for anti-HK2 antibody

1. Expression and role in glycolysis of human ADP-dependent glucokinase. Richter S, Richter JP, Mehta SY, Gribble AM, Sutherland-Smith AJ, Stowell KM, Print CG, Ronimus RS, Wilson WR.Mol Cell Biochem. 2012 Jan 5.

Other Notes

Small volumes of anti-HK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HK2 purchase

MBS6136986 is an antibody targeting HK2. Also known as HK2 (Hexokinase-2, Hexokinase Type II, HK II, HKII, HK2, HXK2, DKFZp686M1669, Muscle Form Hexokinase) APC. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens hexokinase 2, mRNA
NCBI Official Synonym Full Names
hexokinase 2
NCBI Official Symbol
HK2
NCBI Official Synonym Symbols
HKII; HXK2
NCBI Protein Information
hexokinase-2

NCBI Summary for HK2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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